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題名 | 食品中酸性水溶性煤焦色素分析方法之探討 |
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作 者 | 周政輝; 范晉嘉; | 書刊名 | 嘉南學報 |
卷期 | 19 1993.11[民82.11] |
頁次 | 頁45-55 |
分類號 | 412.373 |
關鍵詞 | 煤焦色素; 離子配對層析法; 逆相高效液相層析法; Coal-tar colors; Ion-pair chromatography; Reverse-phase high perfomance liquid chromatography; |
語文 | 中文(Chinese) |
中文摘要 | 本研究乃以離子配對層析法(ion-pair chromatography)配合逆相高效液相 層析法(Reversed phase high performance liguid chromatography)。分離並定量二十種 規定內與規定外之酸性水溶性煤焦色素。並希望能以C18之Sep-pak管柱成功地簡 化前處理步驟,縮短前處理時間,並提高色素之回收率。 經本研究發現Sep-pak管柱幾乎可以完全吸附20種酸性煤焦色素,並且回收率皆在 96以上(除New Coccin外)。而離子配對層析也成功地分離並定量十七種酸性煤焦 色素。經採購市售之蜜餞5件,糖果11件,果凍布丁4件,其它2件,合計22件。 發現約有一半食品與標示不符,但大部份皆使用規定內煤焦色。唯有兩件發現可 能是添加了規定外之Methanil Yellow及Amaranth色素。 |
英文摘要 | The objective of this experiment is to separate and quantify twenty acidic and water-solublecoal-tar colors by extraction with the C18 Sep-pak cartridges and separation with reverse-phasehigh performance liquid chromatography. The results revealed that uses of C18 Sep-pak cartridge could easily absorb all coal-tar colors and simplify the preparation procedure, and all the recovery yields was over 96 (exceptNew coccin). The method of ion-pair chromatography also successfully separated seventeen acidiccoal-tar colors. Twenty-two food samples obtained from the local supermarkets in Tainan, including 5 preserved fruits, 11 candies, 4 jellies and pubings, and two other food samples were examined. Almost hiaf of 22 food samples demonstrated that the food colors used in samples were notaccordant to the description on the package labels of the samples, but most of the food colorswere legally acidic coal-tar colors, except the illegal dyes of methanil yellow and amaranth werefound in two samples. |
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