頁籤選單縮合
題名 | 改善以脫脂乳保存的山羊冷凍精液解凍後之品質=Improving the Post-thawing Quality of Caprine Frozen Semen Cryopreserved with Skimmed Milk Extenders |
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作者 | 吳昇陽; 章嘉潔; 沈朋志; | 書刊名 | 畜產研究 |
卷期 | 48:3 2015.09[民104.09] |
頁次 | 頁188-195 |
分類號 | 437.58 |
關鍵詞 | 山羊; 冷凍精液; 脫脂乳; Goat; Frozen semen; Skimmed milk; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗為改善原以脫脂乳(skimmed-milk, SKM) 製成的山羊冷凍精液解凍後受胎率低之問題。研究以SKM 冷凍精液經解凍後添加不同比例之YTF (egg yolk-tris-fructose, YTF) 稀釋液,對精子性狀之影響。精液性狀評估係應用電腦輔助之精液分析系統與VideoTesT-sperm 2.1 軟體進行。評估項目包括精子活力、精子前進式活力、精子移動能力參數值及頭帽完整率。結果顯示SKM 冷凍精液解凍後添加0.5 倍YTF 稀釋液(1:0.5),體外培養至4 及6 h之精子活力及精子前進式活力,均較無添加稀釋液(1:0) 者呈現顯著之改善(P < 0.05)。然而,是否添加YTF 稀釋液對於精子頭帽完整性方面並無顯著之影響。SKM 冷凍精液分別以無添加稀釋液與添加0.5 倍YTF 解凍稀釋後人工授精之結果,二組之產仔率分別為33.3% 與66.7% (P < 0.05),產仔數分別為4 與15,每頭母羊之平均產仔數分別為1.0 與1.5 頭(P < 0.05)。此等結果顯示以0.5 倍YTF 解凍稀釋添加於解凍後之SKM 山羊冷凍精液,可以改善其解凍後之精子性狀及繁殖性能表現。 |
英文摘要 | The objective of this experiment is to improve the low conception rate of the caprine frozen semen cryopreserved with skimmed-milk. The effects of egg yolk-tris-fructose (YTF) extender addition on the sperm quality of frozen-thawed skimmed-milk (SKM) semen were determined with computer-assisted sperm motility analysis system (CASA) and Semen Analyzer (VideoTesT-sperm 2.1). Frozen-thawed semen was extended in YTF extender at the proportion of 1 : 0, 1 : 0.5 and 1 : 1, respectively. The frozen-thawed semen characteristics including motility, progressive motility, CASA motility and acrosomal integrity were evaluated after extending and cultured for 0, 2, 4 and 6 h at 37˚C. The sperm motility and progressive motility percentage of frozen-thawed SKM semen extended in YTF extender at the proportion of 1 : 0.5 after thawing for 4, 6 h were significantly higher than those of the extended in YTF extender at the proportion of 1 : 0 (P < 0.05). No significant difference among the sperm acrosome integrity of frozen-thawed semen extendedin all different extender compositions. Artificial insemination with frozen-thawed SKM semen extended in YTF extender at the proportion of 1 : 0 and 1 : 0.5 resulted in significantly different kidding rates (33.3% vs. 66.7%; P < 0.05), the number of the kids (4 vs. 15) and the average litter size (1.0 vs. 1.5; P < 0.05). These results indicated that the SKM: YTF proportion of 1 : 0.5 in frozenthawed SKM semen served as an optimal improvement for post-thawing semen quality and reproductive performance. Key words: Goat, Frozen semen, Skimmed milk. |
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