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題 名 | 基因轉殖山羊生產方式簡化研究:採自性週期自然發情山羊之受精卵基因注入後移置回原輸卵管之生存率探討=Research on Simplified Method for the Production of Transgenic Goat: Survival Rate of Microinjected Zygotes Transferred back into the Oviduct of the Same Natural Cyclic Estrous Goat |
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作 者 | 許登造; 姜麗萍; 吳崇興; | 書刊名 | 中國畜牧學會會誌 |
卷 期 | 27:2 1998.06[民87.06] |
頁 次 | 頁243-250 |
分類號 | 437.58 |
關鍵詞 | 山羊; 基因轉置; 輸卵管胚移置; 自然發情; Tubal transfer; Gene transfer; Goat; Cyclic estrus; |
語 文 | 中文(Chinese) |
中文摘要 | 本試驗之目的旨在控討將基因轉殖山羊生產之工作,融入例行性繁殖管理作業, 冀能建立價廉之山羊基因轉殖生產模式。鑒於山羊超級排卵反應較綿羊低,以性週期正常之 自然發情配種後採集之受精卵被以豬乳鐵蛋白基因注入雄原核內,然後立即移置於尚在麻醉 中之同一母山羊輸卵管內,以節省勞力。如兩邊卵巢只有一排卵點則比較移置於排卵管同側 或對側輸卵母山羊所得的受胎率,並與超級排卵母山羊所得的受胎率比較之。試驗結果得知 14頭自然發情山羊之16個受精卵經基因注入,結果有8頭(57%)母山羊受胎,生下10頭(62.5%) 仔羊。十三頭自然發情山羊之15個受精卵,經基因注入後移置於排卵點對例之輸卵管,結 果有4頭母山羊(33%)受胎,出生5頭仔山羊(33%)。以同側輸卵管移置成功率較佳(P<0.05)。 六頭超級排卵處理的母山羊之22個受精卵,經基因注入及移置後均無受胎,七頭母山羊因 多次手術以致生殖器官粘連,無法沖洗胚,但仍能懷孕生下12頭仔羊。試驗結果說明,自 然發情之母山羊之受精卵,經基因注入再移入原輸卵管之處理方式可應用於小型山羊基因轉 殖試驗。 |
英文摘要 | The objective of this study was attempting to simplify the production of transgenic goat by collecting zygotes from natural cyclic estrous goats without synchronization estrus and superovulation. The Taiwanese native goat does were managed under free grazing conditions and were used for zygotes collection and transfer. Goats in estrus were mated twicely by two to three bucks (day 0). Foreign gene ( LA- pLF) was used and zygotes were collected in day 1~3 surgically by flushing 10 ml m-PBS medium through the oviduct from either the uterotubal junction (UTJ) or ishmoampulla junction (AIJ) toward the fimbriae. Microinjected zygotes were transferred back to the oviduct of the donor does still in laparotomy. The viability of embryos transferred to the oviduct ipsilateral or contra lateral to the ovulated ovary was compared. Results of this experiment were as compared. (1)Of the 14 does receiving 16 injected embryos, eight (57%) were pregnant and ten kids (62.5%) were born in the ipsilateral group. Of 13 does receiving 15 injected embryos, four (30.7%) were pregnant and five Kids (33%) were born in the contra lateral group. The difference of survival rate of injected embryos is significant between two groups (P<0.05). (2)Of the 6 superovulated does receiving 22 injected embryos, none were pregnant. (3)Seven does had adhesive uterine horns due to multiple laparotomies but they had the ability to conceive and gave birth of 12 kids. In conclusion, results indicated the rate of kidding was not less than that of the conventional method for production of transgenic goat without the synchronization and super ovulation treatments for donor and recipient does. |
本系統中英文摘要資訊取自各篇刊載內容。