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題名 | 探討不同粒徑SiO₂-Allicin抗菌微粒之吸附量及抑菌效果=Effect of Particle Sizes on the Absorption Capacity and Antimicrobial Activity of the SiO₂-allicin Particles |
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作者 | 劉佳玲; 邱震元; | 書刊名 | 民生論叢 |
卷期 | 10 2014.07[民103.07] |
頁次 | 頁1-12 |
分類號 | 463.1 |
關鍵詞 | 大蒜素; 二氧化矽; 抗菌微粒; 吸附量; 食品包裝; Allicin; Silica; Antimicrobial particles; Absorption capacity; Food packaging; |
語文 | 中文(Chinese) |
中文摘要 | 本研究的目的在探討不同粒徑(<25μm、25~44μm和44~74μm)之SiO_2-Allicin抗菌微粒對allicin的吸附能力,對金黃色葡萄球菌、大腸桿菌的抑菌能力及不同重量(0.02、0.05、0.08和0.1g)下SiO_2-Allicin抗菌微粒對黴菌的抑菌能力,找出最佳抗菌能力的SiO_2-Allicin抗菌微粒,以應用在食品包裝上。實驗方法主要利用分光光度計測定不同粒徑SiO_2-Allicin抗菌微粒中allicin的吸附量。利用SiO_2-Allicin抗菌微粒在培養皿中揮發allicin形成抑菌圈,再由抑菌圈大小換算抑菌率的方式,來探討SiO_2-Allicin抗菌微粒對金黃色葡萄球菌、大腸桿菌及黴菌的抑制能力。實驗結果得到<25μm粒徑的SiO_2-Allicin抗菌微粒對allicin的吸附量為11.09 ± 0.41mg/g,與44~74μm粒徑的抗菌粒子(9.85 ± 0.33 mg/g)及25~44μm粒徑的抗菌粒子(10.18 ± 0.48mg/g)之間有顯著差異(p<0.05)。探討微生物的抑菌能力方面,從大腸桿菌與金黃色葡萄球菌抑菌率來看,大腸桿菌抑菌率為58.91±0.67%,金黃色葡萄球菌為75.58 ± 1.16%,兩者之間有顯著性差異(p<0.05),也就是說allicin對於大腸桿菌的抑制能力小於金黃色葡萄球菌。探討抗菌粒子的量對黴菌的抑制能力來看,SiO_2-Allicin抗菌微粒的量越多,其抑菌能力越強,隨著儲存天數(1~5天)的增加,不同重量(0.02~0.08g)抗菌粒子的抑菌能力逐漸降低,跟對照組比較含有0.1g抗菌粒子的抑菌率,可以維持五天仍為100%。 |
英文摘要 | This study investigated the effect of different particle sizes on the absorption capacity and the antibacterial activity, and the effect of different particle weights on the antifungal activity of the SiO_2-allicin particles. Three sizes (<25 μm, 25-44 μm, and 44-74 μm) of the particles were used to determine the absorption capacity of allicin and antibacterial activity against Staphylococcus aureus and Eschericia coli. Four weights (0.02, 0.05, 0.08, and 0.1 g) of the particles were used to determine the antifungal activity against Aspergillus nige. Results showed that the absorption capacity of allicin obtained in the particle sizes of <25 μm, 25-44 μm, and 44-74 μm were 11.09 ± 0.41 mg/g, 10.18 ± 0.48 mg/g, and 9.85 ± 0.33 mg/g, respectively; there was statistically significant difference (p<0.05). It indicates that the smallest particles had a best absorption capacity. The antibacterial activity of the SiO_2-allicin particles with the size of <25 μm showed that the inhibition rate on Staphylococcus aureus and Eschericia coli were 75.58 ± 1.16% and 58.91 ± 0.67%, respectively; there was a significant difference (p<0.05). It indicates that the SiO_2-allicin particles with the size of <25 μm had a better antibacterial capacity on Staphylococcus aureus than on Eschericia coli. The effect of particle weights on the antifungal activity against Aspergillus niger showed that the more the SiO_2-allicin particles added (0.02-0.08 g), the stronger the antifungal capacity obtained during a 5-day storage. In addition, the antifungal capacity of the 0.02-0.08 g particles decreased as the storage time increased. Compared to the control, the antifungal capacity of the 0.1 g SiO_2-allicin particles remained a 100% inhibition rate up to Day 5. |
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