頁籤選單縮合
題名 | Characterization of Xanthomonas Associated with Bacterial Spot of Tomato and Pepper in Taiwan=臺灣Xanthomonas屬茄科植物細菌性斑點病菌之特性分析 |
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作者 | 呂昀陞; 鄧文玲; 吳雅芳; 鄭安秀; 徐世典; 曾國欽; Lue, Y. S.; Deng, W. L.; Wu, Y. F.; Cheng, A. S.; Hsu, S. T.; Tzeng, K. C.; |
期刊 | 植物病理學會刊 |
出版日期 | 20100900 |
卷期 | 19:3 2010.09[民99.09] |
頁次 | 頁181-190 |
分類號 | 433.4 |
語文 | eng |
關鍵詞 | 細菌素; Xanthomonas euvesicatoria; Xanthomonas gardneri; Xanthomonas perforans; Xanthomonas vesicatoria; Bacteriocin; |
中文摘要 | 細菌性斑點病為茄科植物重要細菌性病害之一,最近引起番茄與甜椒細菌性斑點病之 Xanthomonas 細菌已被重新分類為 Xanthomonas euvesicatoria (Doidge) Jones et al.、X. gardneri (Sutic) Jones et al.、X. perforans Jones et al. 與 X. vesicatoria (Doidge) Vauterin et al. 等四種。為了解臺灣 Xanthomonas 屬茄科植物細菌性斑點病菌之特性與種類,本研究利用生化特性、PCR 反應與碳 素源利用等,分析臺灣各地分離之 53 個茄科植物細菌性斑點病菌菌株,結果顯示其中 40 個菌 株不具澱粉與果膠分解能力,其餘 13 個菌株則同時具有澱粉與果膠分解能力;以 X. euvesicatoria (Doidge) Jones et al. 之專一性引子對 RST 13/14 與 X. vesicatoria (Doidge) Vauterin et al. 之專 一性引子對 C-2-2L/2R 進行多引子聚合酵素連鎖反應 (multiplex PCR) 測試,發現 40 菌株可增 幅出 560 bp 之 X. euvesicatoria 專一性 DNA 片段,5 個菌株可增幅出 407 bp 之 X. vesicatoria 專 一性 DNA 片段,但有8個菌株無法以此 2 組引子對增幅出預期之產物;利用 Biolog 鑑定系統 進行菌株碳素源利用測試,並以 NT-SYS pc 2.0 分析,結果顯示,供試之 53 個 Xanthomonas 菌 株,有 40 株為 X. euvesicatoria,8 株為 X. perforans 及 5 株為 X. vesicatoria。本研究測試之菌 株中並未有 X. gardneri 特性之菌株。此外本研究鑑定為 X. perforans 之菌株,皆可產生對 X. euvesicatoria 菌株生長具抑制作用之細菌素。本篇為臺灣 X. perforans 之首次報導。Xanthomonas perforans 在臺灣之分佈及其生態特性值得進一步探討。 |
英文摘要 | Bacterial spot is an important disease of tomato and pepper in Taiwan. Recently, the strains of Xanthomonas associated with bacterial spot of tomato and pepper were classified into four species, namely X. euvesicatoria (Doidge) Jones et al., X. gardneri (Sutic) Jones et al., X. perforans Jones et al. and X. vesicatoria (Doidge) Vauterin et al.. To characterize Xanthomonas species associated with bacterial spot of tomato and pepper (BSX) in Taiwan, 53 BSX strains collected from various localities in Taiwan were analyzed. Among them, 13 strains were amylolytic and pectolytic. The 560 bp DNA fragment specific for X. axonopodis pv. vesicatoria was amplified from 40 strains with primer pair RST13/14, and the 407 bp DNA fragment specific for X. vesicatoria was amplified from five strains with primer pair C-2-2L/2R, however no products could be amplified from the other eight strains by either primer pairs. Carbon sources utilization of these BSX strains were tested on Biolog GN2 microplate. Three utilization patterns of 13 differential carbon sources were observed as described for X. euvesicatoria, X. vesicatoria and X. perforans. Based on the characterization results, 40 strains of X. euvesicatoria, 8 strains of X. perforans and 5 strains of X. vesicatoria were identified. No X. gardneri strains were found in this study. In addition, the strains identified as X. perforans produced bacteriocins that inhibited the growth of X. euvesicatoria. This is the first report of X. perforans in Taiwan. The distribution and ecological fitness of X. perforans strains in Taiwan is worthy of further study. |
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