頁籤選單縮合
題名 | 利用液相層析串聯質譜儀檢測豬肝中動物用藥殘留之多重分析法=Simultaneous Determination of Multiclass Veterinary Drug Residues in Porcine Liver by Liquid Chromatography-Electrospray Tandem Mass Spectrometry |
---|---|
作者 | 盧信芳; 唐玉芸; 林旭陽; 黃登福; 闕麗卿; 羅吉方; | 書刊名 | 臺灣農業化學與食品科學 |
卷期 | 49:2 2011.04[民100.04] |
頁次 | 頁64-73 |
分類號 | 412.37 |
關鍵詞 | 液相層析串聯質譜法; 巨環類; β-內醯胺類; 林可黴素類; Liquid chromatography tandem mass spectrometry; LC/MS/MS; Macrolides; β-lactam; Lincosamides; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗方法使用液相層析串聯質譜儀(liquid chromatography/tandem mass spectrometry, LC/MS/MS),以電灑游離正離子法,進行豬肝中巨環類(macrolides)抗生素、β-內醯胺類(β-lactam)抗生素、林可黴素類(lincosamides)抗生素及其他類抗生素之方法開發。研究中針對檢測豬肝中殘留15種抗生素之快速多重殘留分析方法進行開發。試驗步驟為肝臟檢體以甲醇、Na2EDTA水溶液及乙腈萃取,以矽藻土淨化之萃取液,經氮氣濃縮後,選用Acquity UPLC HSS T3之層析管柱,搭配0.005%甲酸水溶液與乙腈進行分析。此方法之平均回收率大多介於50.2~115.8%間,平均變異係數為2.8~15.1%,檢測限量介於0.25~10 ppb。本研究提供了一個快速且敏感的多重殘留分析方法,可同時檢測豬肝中15種品項之動物用藥。 |
英文摘要 | We described a method by using liquid chromatography tandem mass spectrometry (LC/MS/MS) combined with positive electrospray ionization to identify different classes of antibiotics (macrolides, β-lactam antibiotics, lincosamides and miscellaneous antibiotics). The aim of this study was to develop a rapid method for simultaneous determination of 15 antibiotics in porcine liver. Sample preparation was performed using liquid/liquid extraction with methanol, Na2EDTA and acetonitrile. The extracts were cleaned up with Hyflo Supercel and were evaporated to dryness under a stream of nitrogen. The chromatography was carried out on a Acquity UPLC HSS T3 column as mobile phase component A was water with 0.005% formic acid, and component B was acetonitrile. The average recoveries were 50.2% to 115.8%, and coefficients of variation were from 2.8% to 15.1%, indicating this method is reliable. Estimated limits of quantification were 0.25-10 ppb. In this paper, a rapid and sensitive multiclass method had been developed on simultaneous determination of 15 antibiotics in porcine liver. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。