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題名 | 利用液相層析串聯質譜法分析化粧品中之雌激素=Determination of Estrogens in Cosmetics by Liquid Chromatography-Tandem Mass Spectrometry |
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作者 | 陳信豪; 黃守潔; 陳玉盆; 闕麗卿; 施養志; | 書刊名 | 食品藥物研究年報 |
卷期 | 5 2014.12[民103.12] |
頁次 | 頁299-306 |
分類號 | 412.36 |
關鍵詞 | 液相層析串聯質譜法; 雌激素; 化粧品; LC/MS/MS; Estrogen; Cosmetics; |
語文 | 中文(Chinese) |
中文摘要 | 本研究係採用液相層析串聯質譜法(liquid chromatography-tandem mass spectrometry, LC/MS/ MS),以正/負離子電灑法(positive/negative mode)之多重反應偵測(multiple reaction monitoring, MRM)模式同時檢測化粧品中Estriol、Estradiol、Estrone、Ethinyl estradiol、Diethylstilbestrol、 Pregnanediol、Progesterone及Estradiol benzoate 8種雌激素(Estrogens)成分之檢測方法。使用之層析 管柱為 Waters UPLC BEH C18 (1.7 μm, 內徑2.1 mm × 100 mm) ;移動相為去離子水和乙腈進行梯 度流洗;流速為0.3 mL/min;本研究方法中針對8種雌激素成分檢測,可在10分內鐘完成分析,標 準曲線之線性範圍為0.005-1.0 μg/mL,標準曲線線性回歸係數r2皆為0.9953以上。添加不同濃度之 標準品於空白乳液中進行三重複試驗,回收率介於85.2-115.0%,相對標準偏差均小於8.8%。 |
英文摘要 | A method for the analysis of 8 estrogens in cosmetics by liquid chromatography-tandem mass spectrometry (LC/MS/MS) with electro spray ionization was developed. Data acquisition under MS/MS for each estrogen was achieved by applying multiple reaction monitoring (MRM). Estriol、estradiol、 ethinyl estradiol、estrone、diethylstilbestrol、progesterone and estradiol benzoate were extracted from cosmetics with methanol, and analyzed by LC/MS/MS with electrospray ionization.The LC separation was conducted on an Waters ACQUITY BEH C18 (I.D. 2.1 mm × 100 mm, 1.7 μm) column with gradient elution of water and acetonitrile as the mobile phase. The concentration range of standards were 0.005- 1.0 μg/mL with an r2 value greater than 0.9953. The average recoveries of the 8 estrogen spiked in instant creams ranged from 85.2-115.0% and the relative coefficients of variation of all estrogens were below 8.8%. |
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