頁籤選單縮合
題 名 | 藥用植物苦參微體繁殖及瓶苗馴化研究=Micropropagation and Acclimation Studies on Medicinal Plant of Sophora flavescens Ait. |
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作 者 | 林資哲; 夏奇鈮; 陳威臣; 葉茂生; | 書刊名 | 作物、環境與生物資訊 |
卷 期 | 7:3 2010.09[民99.09] |
頁 次 | 頁145-152 |
分類號 | 434.92 |
關鍵詞 | 苦參; 藥用植物; 微體繁殖; 馴化; Sophora flavescens Ait.; Medicinal plant; Micropropagation; Acclimation; |
語 文 | 中文(Chinese) |
中文摘要 | 摘要 藥用植物苦參(Sophora flavescens Ait.)大多生長於野外,天然結實之種子發芽不易,臺灣目 前亦少有商業形式的栽培,市場所需藥材皆仰賴中國大陸進口。臺灣如欲大量栽種苦參,必須 克服種子發芽率低之問題外,亦可利用組織培養技術建立苦參大量繁殖方法,以提供商業栽培 所需之種苗。本研究以花蓮縣生產之苦参種子建立無菌瓶苗,再以瓶苗進行芽體增殖、瓶苗發 根及瓶苗出瓶馴化等試驗。以兩種方法比較促進苦參種子發芽效果,一以1% HCl 溶液浸泡種 子30 min,另一以 60℃溫水浸泡種子60 min。在無菌播種後的結果顯示,以60℃溫水處理之 苦參種子,接種在1/2 MS 鹽類配方培養基中,發芽率最高可達73.33%。瓶苗繁殖方面,以莖 節之瓶苗莖段為培植體,接種於添加0-2.0 mg L-1 BA (benzyladenine) 或 TDZ (thidiazuron) 之 MS 基礎鹽類培養基中培養,結果以 2.0 mg L-1 BA 處理組之芽體誘導率最高(96.67%),平均 每個莖段可增殖10.4 個芽體。瓶苗發根方面,待芽體生長至1.5-2 cm 後,切取單一芽體接種 在添加0-1.0 mg L-1 NAA (α-naphthalenacetic acid)或IBA (indole-3-butyric acid)之 1/2 MS 培養基中誘導發根,結果以1.0 mg L-1 NAA 處理之瓶苗具最高之根數及根長表現。將已發根 高約 4-5 cm 之組培苗移出瓶外種植,調查瓶苗期間施以不同濃度NAA 與IBA 之發根植株, 經過2 及4 wks 馴化處理後的生長情形,結果顯示,瓶內發根次佳之處理 (0.5 mg L-1 NAA) 在 經4 wks 馴化後其小苗生長較瓶內發根最佳之1 mg L-1 NAA 處理表現更佳。顯見在瓶內發根 階段,雖然較高濃度的auxin 處理下有利於根的生長,但過高之生長素濃度可能不利於後續植 株之生長。因此,建議在瓶內發根培養時使用較低之auxin 濃度即可,對於出瓶後小苗之生長 可能更為有利。 |
英文摘要 | ABSTRACT Sophora flavescens Ait. is a commonly used medicinal plant in Taiwan. However, roots of S. flavescens for medicinal needs are mostly imported from China. Therefore, studies on how to overcome the problem of low seed germination and provide sufficient seedings of S. flavescens for commercial cultivation were carried out. Seeds of S. flavescens were collected from Hualien Hsien of Taiwan and were used to establish aseptic explants, shoot induction, proliferation, rooting and ex vitro acclimation. Two methods were examined for the function in increasing seed germination. In the first one, seeds were soaking in 1 % HCl solution for 30 min, and the second one used 60℃ warm water for 60 min. The germination rate went up to 73.33% on a 1/2 MS- based salt medium in the second method. Nodal explants were cultured in a MS-based salt medium containing 0-2.0 mg L-1 BA (benzyl- adenine) or TDZ (thidiazuron) for shoot proliferation. The highest induction rate (96.67%) was obtained at 2.0 mg L-1 BA with 10.4 shoots explants-1. Shoots with 1.5-2.0 cm in length were subcultured into a 1/2 MS-based salt medium containing 0-1.0 mg L-1 NAA (α-naphthalenacetic acid) or IBA (indole-3-butyric acid) for rooting. Both the highest root number and root length were obtained at 1.0 mg L-1 NAA treatment. Seeding growth after 2 to 4-weeks ex vitro acclimation was recorded. After 4-week acclimation, the second best rooting treatment in vitro (0.5 mg L-1 NAA) was found superior than 1 mg L-1 NAA treatment. Although higher auxin treatment might be benifical to in vitro rooting, a lower concentration of auxin for ex vitro seeding growth was recommended. |
本系統中英文摘要資訊取自各篇刊載內容。