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題名 | 唐菖蒲球莖微體繁殖與馴化之研究=Studies on the Micropropagation and Acclimatization of Gladiolus Hybridus Hort |
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作者姓名(中文) | 陳美滿; 王才義; | 書刊名 | 興大園藝 |
卷期 | 22:2 1997.12[民86.12] |
頁次 | 頁113-121 |
分類號 | 435.452 |
關鍵詞 | 唐菖蒲; 微體繁殖; 馴化; Gladiolus; Micropropagation; Acclimatization; |
語文 | 中文(Chinese) |
中文摘要 | 唐菖蒲球莖莖頂培植體培養於不同濃度 BA 的 MS 培養基中, 品種間芽體的增殖 率,以′ Pink Lady ′增殖率最高。 一般培植體隨著 BA 濃度增加,而產生大量短縮化芽 體或形成癒合組織。 培養基中添加 BA 芽體增殖率較添加 Kinetin 的處理效果佳。培植體 於誘導發根期, 以半量 MS 培養基中添加活性碳 3 mg/l、NAA 0.5 mg/l,提高培養基中蔗 糖濃定,可提高瓶苗的根長、根數、瓶苗鮮重、瓶苗乾重及移植後的存活率。瓶苗至形成小 球莖,由二途徑,一為瓶苗經馴化後移植於溫室中,於溫室中形成小球莖;另可將瓶苗培養 於瓶內,至形成小球莖,前者所形成之小球莖明顯較後者大。 |
英文摘要 | The explants are cultured in MS medium to which the different concentration of BA are added. As a result, "Pink Lady" has the greatest multiplication rate. In general, the explants produce increasingly the stunt shoots or callus in proportion to the increase of the BA concentration. The multiplication rate in the MS medium with supplement BA in more than that supplement Kinetin. During the period of inducing the roots of the explants, if the activated charoal 3 g/l, NAA 0.5 mg/l and higher sucrose concentration are added to the 1/2 MS basal medium, it will help the development root length, roots number, fresh weight of plantlets, dry weight of plantlets, and enhance the survival rate after transplanting. Plantlet produced cormlets two ways, one transplanted plantlets, after acclimatization, produced cormlets in greenhouse, the other plantlets produced cormlets in vitro. The former produced larger-size cormlets then the latter. |
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