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題 名 | 湖北貝母組織培養之研究(3)--培養基組成分及光照處理對誘導小鱗莖發根之影響=Studies on the Tissue Culture of Fritillaria hupehensis Hsiao et K. C. Hsia (Ⅲ)--Influence of Medium Component and Light Treatment on in vitro Rooting, and Acclimatization of Bulblet |
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作 者 | 陳威臣; 戴宗德; 陳忠川; 蔡新聲; | 書刊名 | 中華農業研究 |
卷 期 | 49:4 2000.12[民89.12] |
頁 次 | 頁39-47 |
分類號 | 434.92 |
關鍵詞 | 湖北貝母; 藥用植物; 組織培養; 瓶內發根; 馴化; Fritillaria hupehensis; Medicinal plant; Tissue culture; In vitro rooting; Acclimatization; |
語 文 | 中文(Chinese) |
中文摘要 | 貝母(Bei-Mu)首載於神農本草經、草部中品,為傳統中醫藥用植物,依 藥用習慣分為浙貝母和川貝母兩大類。浙貝母苦寒,多用於外感咳嗽;川貝母苦 甘微寒,多用於虛勞咳嗽。在湖北西部和四川東部所發展的湖北貝母(Fritillaria hupehensis Hsiao et K. C. Hsia),屬川貝母之一種。湖北貝母具止咳化痰之療效, 歷年來均採鱗莖繁殖播種,但繁殖速率低、產量少,不能滿足醫療之需。本研究 乃探討利用組織培養大量繁殖湖北貝母種苗之技術。將培養湖北貝母鱗片所獲得 之小鱗莖,繼代培養在不同濃度的NAA培養基中,結果發現4 mg/1 NAA (α- naphthaleneacetic acid)誘導小鱗莖發根之比率最高,可達63.3%,隨NAA濃度降 低而小鱗莖而小鱗莖發根比率漸少;每個小鱗莖之發根數目則以2 mg/1 NAA較 佳。將小鱗莖培養在 1/2 MS 基本鹽類濃度、3%蔗糖,配合0.5 mg/1 BA (N6- benzyladenine)及 4 mg/1 NAA之固體培養基中,於光照16小時/日、2,000 lux光 強度,25℃恆溫的條件下培養60天後,約65%之鱗莖可得良好根系。將形成良 好根系之小鱗莖移出試管,栽培於土壤、泥炭苔與河砂(v/v/v=2/1/1)之混合介質, 置於生長箱內進行馴化,一個月後之植株成活率可達90%。 |
英文摘要 | Bei-Mu (Fritillaria hupehensis Hsiao et K. C. Hsia) has been used as antiussive and expectorant in traditional Chinese medicine. As the amount of bulb collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, therefore the mass propagation method using bulblets of F. hupehensis has been established. α-Naphthaleneacetic acid (NAA) in concentration range of 2-4 mg/l could promote the root initiation of the bulblets. The bulblets cultured on medium containing 1/2 Murashige and Skoog (MS) medium, 3% sucrose, 0.5 mg/l 6-benzylaminopurine (BA), and 4 mg/l NAA for 60 days, consequently, 65% bulblets could develop good root system. The in vitro propagated bulblets with well-developed roots could be transplanted to autoclaved soil, peat moss, and sand (v/v/v=2/1/1), and grown well in a growth chamber. The survival percentage of bulblets was 90% by one month of culture. |
本系統中英文摘要資訊取自各篇刊載內容。