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題 名 | 湖北貝母組織培養之研究=Studies on the Tissue Culture of Fritillarua Hupehensis Hsiao et K. C. Hsia |
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作 者 | 蔡新聲; | 書刊名 | 中醫藥年報 |
卷 期 | 15:2 1997.05[民86.05] |
頁 次 | 頁785-809 |
分類號 | 414.34 |
關鍵詞 | 湖北貝母; 芽球相似體; 組織培養; Fritillaria hapehensis hsiao et K.C.Hsia; Protocorm like body; Tissue culture; |
語 文 | 中文(Chinese) |
中文摘要 | 將葉片、莖段基部、鱗片及PLB(芽球相似體)四種培植體,培養於含有不同濃度BA之培養基中,結果發現再生鱗片之誘導率以鱗片52.92%最高,莖段基部 41.43%次之,28.24%又次之,而以葉片4.02%最差;而培養60天後之褐化死亡率則相反,以葉片94.78%最高,莖段基部34.04%次之,鱗片及PLB之褐化死亡率較少,分別為16.88% 及13.73%。每個成活培植體形成新鱗片數目則以莖段基部平均5.01個最佳,其於依次為鱗片3.74個,葉片3.2個及PLB 2.58個;而擬胚化癒合組織之誘導則以PLB及莖段基部31.37%及28.72%較佳,其次為鱗片19.48%,而以葉片1.20%最差。衡諸新鱗片誘導率、形成新鱗片數目及擬胚化癒合組織形成率,則以含0.5-2 mg/l BA之培養基較為適當。此外,添加不同濃度之 NAA 可提高新鱗片誘導率(莖段基部由41.43%提高為65.22%;鱗片由 52.92%提高為68.10%),卻不利於形成新鱗片之數目(莖段基部由5.01個下降為3.01個;鱗片由3.74個下降為3.33個);NAA且有誘導培植體發根之作用,在0-4mg/l NAA之範圍內,隨濃度升高而發根漸多,因此較適合湖北貝母培植體培養,誘導新鱗片形成之培養基為0.5-2 mg/l BA配合0.5 mg/l NAA之MS基本鹽類培養基;四種培植體之再生能力順序為:鱗片>莖基部>PLB>葉片。0.5-l mg/l NAA配合0.5mg/l BA之培養基,對PLB及鱗片之增重效果最佳,經60天培養後之PLB及鱗片,可增加重量約15倍。減少或升高NAA濃度,均使PLB及鱗片之增重效果降低。0.5-4mg/1 2,4-D 對誘導鱗片形成新鱗片之能力NAA相仿,但8 mg/l 2,4-D則顯現濃度過高之害;對擬胚化癒合組織之誘導則NAA優於2,4-D;而對鱗片形成根之能力則0.5-8 mg/1 2,4-D均顯現抑制現象;照光與否對新鱗片之形成影響不大。 |
英文摘要 | Be-Mu (Fritillaria hupehensis) was first recorded in Shen-Nung-Pen-Ts'ao-Ching under the middle herb catogory and was recorded in successive Pen-ts'aos of the descending dynasties. It has been used as antitussive and expectorant for the care of coughing and the prevention of phlegm formation. The component peiminine in the bulb of Be-Mu has been suggested as the major active ingredient. As the amount of bulb collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, the tissue culture method of mass propagation was therefore studied. Cytological and phytochemcial study were also conducted for chromosome counting and active ingredient determination. The results are summarized as follows. 1.For the comparison on the efficiency of new bulbscale production among various explants cultured on solid medium containing MS basic salts and different BA concentration for 60 days, bulbscale explant showed the highest percentage (52.92%) followed by stem base (41.43%), protocorn-like-body (PLB) (28.24%) and leaf blade (4.02%) On the contrary, the order of browning percentage during the culturing period was reversed for the four explants. The stem base explant could produce the highest number of bulbscale (5.01 per culture), followed by bulbscale explant (3.74),leaf blade (3.20), and PLB (2.58). The induction percentages of embryogenic callus were higher for PLB and stem base (31.37% and 28.72%, respectively) and lower for bulbscale and leaf blade (19.48% and 1.20%, respectively) 2.The addition of NAA to the medium resulted in increased efficiency of bulbscale formation, e.g., from 41.43% to 65.22 % for stem base explant and 52.92% to 66.10% for bulbscale explant, and concurrently decreased number of new bulbscale produced per culture, e.g., from 5.01 to 3.1 for stem base and 3.74 to 3.33 for bulbscale explant. NAA in the concentration range of 0-4 mg/l could also promote the rooting ability of the explants. 3. Experimental results indicated that medium containing MS basic salts with 0.5-2.0 mg/l BA and 0.5 mg/l NAA was suitable for bulbscale production in vitro. The regeneration ability of explants was highest for bulbscale, followed by stem base, PLB, and leaf blade. 4.A 15-fold increase in fresh weight was recorded on PLB and bulbscale cultured on medium containing MS basic salts supplemented with 0.5-1 mg/l NAA and 0.5 mp/l BA for 60 days. Change of NAA concentration could result in adverse effect on fresh weight gain. 5.Addition of 0.5-4 mg/l 2.4-D to medium showed similar effect on bulbscale production from cultured bulbscale explant as compared to effect of NAA. Phytotoxicity was observed when concentration of 2,4-D was increased to 8 mg/1. The rooting ability of bulbscale was also inhibited by 0.5-8 mg/1 2,4-D. NAA was superior to 2,4-D in the induction of embryogenic callus. Lighting was not necessary for the production of new bulbscale during the culture process. |
本系統中英文摘要資訊取自各篇刊載內容。