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題 名 | 植物生長調節劑及培養基型態對湖北貝母鱗片培養衍生芽球相似體及小鱗片增重之影響=Effects of Plant Growth Regulators and Status of the Medium on Induction and Proliferation of Protocorm-Like-Bodies and Bulblets from Bulbscale Culture of Fritillaria Hupehensis Hsiao et K.C. Hsia |
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作 者 | 楊淑如; 戴宗德; 陳忠川; 蔡新聲; | 書刊名 | 中華農學會報 |
卷 期 | 2:5 2001.10[民90.10] |
頁 次 | 頁414-422 |
分類號 | 434.92 |
關鍵詞 | 貝母; 小麟片; 湖北貝母; 中草藥; 大量繁殖; 癒合組織; 芽球相似體; Bei-mu; Bulbscale; Fritillaria hupehensis; Medicinal herb; Micropropagation; Callus; Protocorm-like-body; |
語 文 | 中文(Chinese) |
中文摘要 | 湖北貝母在植物分類學上屬百合科(Liliaceae)、貝母屬(Fritillaria)之多年生草本植物,多以地下鱗莖(Bulbscale)入藥,為重要且廣泛使用之中藥,歷年來均以鱗莖繁殖,但因鱗莖繁殖倍率低,種子繁殖又而4~5年之生長週期,因此造成藥品嚴重缺乏,不足以滿足醫療之需。本研究以組織培養方式繁殖湖北貝母,除了希望能達到大量繁殖之冒的外,也其望對湖北貝母由鱗莖所誘導芽球相似體(protocorm-like-body, PLB)及小鱗片(bulblet)重量之增加有所助益,以有益於藥用資源之開發與利用。結果顯示,將湖北貝母鱗莖培養在含有0.5mg/l kinetin之基本MS固體培養基中,可誘導形成含PLB及小鱗片(bulblet)之團塊(OLB mass)及癒合組織;而添加適量之2,4-D或NAA於含有0.5mg/l kinetin之基本MS固體培養基中,對PLB mass及癒合組織之形成有促進之效果,NAA之效果又優於2,4-D,其中尤以2~4 mg/l NAA之效果最佳。基本MS培養基添加植物生長調節劑對湖北貝母芽球相似體及小鱗片增重之影響,則以0.5~1.0 mg/l NAA配合0.5 mg/l BA效果最好。進一步再以添加0.5mg/l BA及0.5mg/l NAA 的基本MS培養基,測試固體或液體培養基對湖北貝母芽球相似體及小鱗片增重之影響,結果發現液體培養之效果優於固體;在容量為125ml之三角瓶加入5、10或15ml液體培養基之三種處理中,在60rpm速率振盪下之增重效果以5ml之處理最佳,芽球相似體及小鱗片經培養30天後的增重倍率分別為19.6倍與23.7倍。 |
英文摘要 | Fritillaria hupehensis (Liliaceae), commonly known as Bei-mu in Chinese, is one of the important traditional Chinese medicinal plant. It is propagated by bulbscales. As the amount of bulbscales collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, we have optimized a method of rapid mass progagation of Bei-mu using bulbscales. Callus and protocorm-like-bodies (PLB) were induced by culturing the bulbscales on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 6-furfurylamoino purine (kinetin). PLB mas when cultured on MS medium with either 0~8 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) or (-naphthaleneacetic acid (NAA)) in combination with 0.5 mg/l kinetin proliferated producing bulblets and callus. NAA was found to be superior to 2,4-D in both PLB mass proliferation and callus formation. PLB mass was cultured on MS medium supplemented with 0.1~0.4 mg/l NAA in combination with 0.5 mg/l 6-benzylaminpure (BA) for further proliferation and increase in the fresh weight of PLB was recorded after60 days of culture in light. The PLB mass and bulblets were also cultured in liquid MS medium supplemented with 3% sucrose, 0.5 mg/l BA and 0.5 mg/l NAA in 125-ml Erlenmeyer flasks with 5~15 ml medium. The amount of liquid medium and state of the medium (liquid vs. solid) were found to influence the proliferation of PLB mass and bulblets. |
本系統中英文摘要資訊取自各篇刊載內容。