查詢結果分析
相關文獻
- Antioxidative Effect of Lactic Acid Bacteria: Intact Cells vs. Intracellular Extracts
- 乳酸菌流動層乾燥技術及其應用
- 燻煙及亞硝酸鈉對常溫貯藏去皮雞腿製品化學特性及肉毒桿菌毒素之影響
- 乳酸菌胞外多醣之組成與功能
- 茵陳蒿湯之抗氧化作用的研究
- Effects of the Concentration of Nucleoside Triphosphates on the Transcription of Bacteriophage T7 RNA Polymerase in the Linearized and Supercoiled DNA Templates
- "益生菌"對家禽的功效與作用機制
- Lactobacillus delbrueckii subsp. bulgaricus所含亞麻油酸異構酶之分離與定性
- Hypocholesteremic Ability of Lactobacillus Acidophilus Tested in an in Vitro Broth System
- A Highly Sensitive and Simple Method of Genomic Subtraction
頁籤選單縮合
題名 | Antioxidative Effect of Lactic Acid Bacteria: Intact Cells vs. Intracellular Extracts=乳酸菌之抗氧化作用:菌體與胞內物抗氧化能力之比較 |
---|---|
作者 | 歐珠琴; 呂聰明; 蔡肇基; 顏志恒; 陳暉雯; 林美吟; | 書刊名 | Journal of Food and Drug Analysis |
卷期 | 17:3 2009.06[民98.06] |
頁次 | 頁209-216+231 |
分類號 | 368.87 |
關鍵詞 | 乳酸菌; 抗氧化作用; 低密度脂蛋白氧化作用; 菌體; 胞內物; Lactic acid bacteria; Antioxidation; LDL oxidation; Intact cells; Intracellular extracts; |
語文 | 英文(English) |
中文摘要 | 本研究比較兩株乳酸菌Bifidobacterium longum 和Lactobacillus delbrueckii subsp. bulgaricus 其菌體及胞內物的抗氧化能力。結果顯示,B. longum 和L. delbrueckii subsp. bulgaricus 在109 CFU/mL 時,菌體及胞內物具有清除DPPH 自由基能力(70.4-75.1%)、抑制liposome 過氧化(25-31%)以及顯著降低 Intestine 407 腸細胞脂質過氧化物(malondialdehyde, MDA)的生成。此外,本研究再進一步利用這兩株具有抗氧化能力乳酸菌的活菌體及其胞內物,探討對腦中風病人(cerebrovascular accident, CVA)和健康受試者血液中低密度脂蛋白(LDL)氧化作用的影響。LDL 的氧化作用是以監測LDL conjugated dienes 形成的起始時間(lag time)為指標。當B. longum 和L. delbrueckii subsp. bulgaricus 以109 cfu/mL 的菌數分別處理LDL 時,LDL 氧化作用的起始時間顯著被延長,健康受試者血液中LDL 氧化被抑制效果優於CVA 病人,而當以B. longum 和L. delbrueckii subsp. bulgaricus 109 CFU/mL 菌數的胞內物處理CVA 病人及健康受試者的LDL 時,LDL 氧化作用的起始時間顯著被延長超過180 分鐘,此結果表示,抑制LDL 氧化作用以乳酸菌的胞內物優於菌體。推論乳酸菌的胞內物具有效的抑制因子。 |
英文摘要 | The present study compared the anti-oxidative ability of intact cells and intracellular extracts of two lactic acid bacterial strains, Bifidobacterium longum and Lactobacillus delbrueckii ssp. bulgaricus. Results showed that both intact cells and intracellular extracts of 109 cells of B. longum and L. delbrueckii ssp. bulgaricus had the ability to scavengeα-diphenyl-β-picrylhydrazyl (DPPH) free radical by 70.4-75.1%, to inhibit liposome peroxidation by 25-31%, and to decrease significantly the malondialdehyde (MDA) production in Intestine 407 cells. The effect of intact cells and intracellular extracts of these two bacterial strains on the oxidation of low density lipoprotein (LDL) isolated from cerebrovascular accident (CVA) patients and healthy subjects was also compared. Oxidation of LDL was monitored by measuring the lag time for the formation of conjugated dienes in isolated LDL particles. When LDL was treated respectively with 109 intact cells of B. longum and L. delbrueckii ssp. bulgaricus, the lag time of oxidation of LDL was prolonged significantly. The extent of inhibition was greater on LDL isolated from healthy subjects than from CVA patients. When LDL from either CVA patients or healthy subjects was treated with intracellular extracts of 109 cells of B. longum and L. delbrueckii ssp. bulgaricus, respectively, the copper-mediated oxidation was extensively inhibited with a lag time exceeding 180 min. Results from this study show a greater inhibitory effect on LDL oxidation exerted by the intracellular extract than the intact cells, suggesting the presence of effective inhibitory factors in the intracellular extract. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。