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來源資料
頁籤選單縮合
題 名 | Effects of the Concentration of Nucleoside Triphosphates on the Transcription of Bacteriophage T7 RNA Polymerase in the Linearized and Supercoiled DNA Templates=噬菌體RNA聚合酶在不同濃度的鹽類和核苷酸之下的轉錄終止反應 |
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作 者 | 鄭石通; 李孝偉; | 書刊名 | Taiwania |
卷 期 | 43:4 1998.12[民87.12] |
頁 次 | 頁295-306 |
分類號 | 364.21 |
關鍵詞 | 超螺旋狀DNA; 噬菌體T7RNA聚合酶; Supercoiled DNA template; Bacteriophage T7 RNA polymerase; |
語 文 | 英文(English) |
DOI | 10.6165/tai.1998.43(4).295 |
中文摘要 | 在DNA模板上,thr attenuator是一個含GC逆對稱尾一串T的構造,也是一個 rho-independent的終結子。在此報告中,以在線性狀和超螺旋狀DNA載體中的thr attenuator為測試模式,來分析和比較噬菌體T7 RNA聚合�t,在不同的核��酸濃度之下的 轉錄反應。結果顯示,在低濃度的核��酸下,thr attenuator 無論在線性狀和超螺旋狀 DNA載體,都能阻止較多的噬菌體T7聚合�t進行轉錄,而與線性狀DNA載體比較,噬菌體T7 RNA聚合�t在超螺旋狀DNA載體的thr attenuator上,顯示出較高的較錄終止現像。此外, 在ATP的濃度限制在5uM到150uM之間時,T7 RNA聚合�t所產生的轉錄產物,較其在其它核�� 酸下所產的轉錄產物,多出至少兩倍,這表示ATP可能和GTP競爭於影響T7 RNA聚合�t的轉錄 。 |
英文摘要 | The thr attenuator is a DNA element containing a G+C-rich dyad symmetry followed by a run of poly(dT), and is reported to be a rho-independent terminator. The effects of the concentrations of nucleoside triphosphates on the transcription of bacteriophage T7 polymerases were analyzed at the thr attenuator in the supercoiled and linearized DNA templates. T7 RNA polymerase showed high termination efficiency in the low concentration of nucleoside triphosphates at the thr attenuator in the both linearized and supercoiled templates. The thr attenuator inthe supercoiled DNA template always caused more T7 RNA polymerase to cease transcription than that in the linearized DNA template. Also, when the concentration of ATP is limited from 5 uM to 150 uM, the transcript production by T7 RNA polymerase was at least two folds more than that in the limited supply of any other nucleoside triphosphates. This indicates that ATP may compete with GTP in affecting transcription of T7 RNA polymerase. |
本系統中英文摘要資訊取自各篇刊載內容。