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題 名 | A Simple and Fast Method for Arginine Determination in Grape Juice=離子交換樹脂分離葡萄之中L-精胺酸的快速測定法 |
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作 者 | 李華; 梁新紅; 馮麗丹; 劉延琳; 王華; | 書刊名 | 藥物食品分析 |
卷 期 | 16:3 2008.06[民97.06] |
頁 次 | 頁53-58+91 |
分類號 | 412.37 |
關鍵詞 | 葡萄汁; L-精胺酸; 離子交換; 胺基甲酸乙酯; 阪口反應; Grape juice; Arginine; Ion-exchange; Ethyl carbamate; Sakaguchi reaction; |
語 文 | 英文(English) |
英文摘要 | Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, a known carcinogen. The aim of this study was to develop a simple, fast and accurate method for determining arginine in grape juice with Sakaguchi reaction following the separation of arginine with Amberlite IR120 strong cation-exchange resin. Measurement was done under optimized conditions as follows: sample (mg) to cation-exchange resin (g) ratio of 0.683, sample pH 2.0, sample flow rate 0.5 mL/min, use of distilled water rinsing to avoid interference of other compounds adsorbed on the resin, and elution with 1 mol/L of NaOH solution. This method can be applied in a quick and accurate quantification of arginine in grape juice for controlling the ethyl carbamate level in wine. |
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