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題名 | 紫背脈葉蘭種子發育與發芽之研究=Studies on Seed Development and Germination of Nervilia Plicata (Andr.) Schlter |
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作者 | 林珈芝; 葉茂生; Lin, Chia-chih; Yeh, Mau-shing; |
期刊 | 作物、環境與生物資訊 |
出版日期 | 20080900 |
卷期 | 5:3 2008.09[民97.09] |
頁次 | 頁159-170 |
分類號 | 434.92 |
語文 | chi |
關鍵詞 | 紫背脈葉蘭; 一點癀; 種子發育; 無菌播種; 發芽; Nervilia plicata; Seed development; Aseptic seeding; Germination; |
中文摘要 | 摘要 紫背脈葉蘭為臺灣地區珍貴的藥材,藥 效與金線連相似,但因民間濫採且未加復 育,已日漸稀少。本研究調查其蒴果生長與 種子發育的情形,進而利用未熟種子進行無 菌播種,以探討其發芽的最適條件,獲得以 下結果。紫背脈葉蘭花朵於授粉後1 天子房 即迅速增長且膨大發育成蒴果,約10~14 天,蒴果長度及寬度生長趨緩,大致呈拋物 曲線生長,授粉後第14 天蒴果13.84 mm × 7.43 mm,切開蒴果,其內部部分種子飛散, 與胎座組織分離,以醋酸洋紅染色後種子呈 長橢圓形,胚呈橢圓形,細胞分裂旺盛,其 他細胞已無法染色,停止分裂,細胞核消失。 授粉後第16 天,蒴果13.96 mm × 7.74 mm,幾乎不再生長,切開時內部種子飛散, 除胚外,其他部位已無法染色,但16 天之蒴 果開始裂開。以12~18 天不同成熟度的種子 播種於1/2 MS 培養基,結果14 天者發芽率 12.5 %,16 天者17 % 最高。以15 天之種子 播種在1/2 MS 添加0、0.5、1、2 g L-1 peptone,及30 g L-1 蔗糖,10 g L-1 agar 之 培養基,培養3 個月後,結果以含0.5 g L-1 peptone 者之發芽率最高,達52.8 %,次為 含1 g L-1 peptone (50.0 %)。將幼苗繼代培養 在原培養基3 個月後結果添加0.5 g L-1 peptone 者幼苗之生長最佳,平均每個幼苗 能產生2.67 個球莖,球莖平均直徑長7.9 mm 最大。 |
英文摘要 | ABSTRACT Nervilia plicata (Andr.) Schlter is an important medicinal herb in Taiwan. The medical efficacy of this species is similar to Anoectochilus formosanus. Due to the over harvest, its population in nature has decreased rapidly. In this study, the growth of capsules, development of seed, and germination of seed were investigated so as to establish the aseptic seeding system for propagation. The results were summarized as follows. The growth of capsules in the initial phase was faster than the late phase, fitted to a parabolic curve. By using capsules of different maturing stages, results showed that the 15-16 days old capsules had the better germination. The medium supplemented with 0.5 g L-1 peptone had the best germination (52.8%). Seedings subcultured with the medium of the 0.5 g L-1 peptone had the best growth. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。