查詢結果分析
相關文獻
- 臺灣泡桐花粉發芽與試管內授粉
- 柿花粉發芽研究及人工授粉對果實發育之影響
- Toxicity of Pesticides to Pollen Germination of 'Haden' Mango
- 速生樹種木材製造建築用粒片板之適用性(II-1):柳杉、臺灣泡桐及木油桐製造長薄片型與方薄片型粒片板
- Properties of Wood-based Composites Laminated with Aluminum Plates
- 定向粒片板之超音波傳波特性
- 臺灣油杉空粒種子形成原因的探討
- 荔枝(Litchi Chinensis Sonn)花粉生體外發芽特性之研究
- 仙人掌紅龍果(Hylocereus Undatus Britt.& Rose)花粉形態與活力之研究
- Nodule Cultures of Paulownia X Taiwaniana
頁籤選單縮合
題 名 | 臺灣泡桐花粉發芽與試管內授粉=Pollen Germination and in Vitro Pollination of Paulownia x Triwaniana |
---|---|
作 者 | 何政坤; 張淑華; 陳振榮; 蔡錦瑩; | 書刊名 | 臺灣林業科學 |
卷 期 | 13:2 1998.06[民87.06] |
頁 次 | 頁127-138 |
分類號 | 436.259 |
關鍵詞 | 臺灣泡桐; 試管內授粉; 花粉發芽; In vitro pollination; Paulownia x triwanian; Pollen germination; |
語 文 | 中文(Chinese) |
中文摘要 | 台灣泡桐花粉的發芽率隨著花苞的發育而提高,同時對蔗糖濃度需求 也呈現差異。當休眠花苞突破休眠而抽長至30 mm長時,花粉開始具有發芽力, 且必須在含有蔗糖的BK培養液才能發芽。當花苞開放後,花粉則可在含或不含 有蔗糖的BK培養液中發芽,且發芽率高達95 %。開花時期尚未裂開的花藥經 表面消毒後,可獲得同樣高發芽率的無菌花粉,可作為試管內授粉之用。胎座授 粉是在花開期的4核胚囊,而雌蕊授粉的發育期為2核胚囊。經授粉結果顯示: 授粉雌蕊培養在不添生長激素的MS培養基,可使雌蕊內25 %的胎座受精,但 受精胚的形成率以培養在MS培養基添加0.5 mg/l zeatin與0.5 mg/l IAA的胎座 最好,每個胎座可獲得100個受精胚。而胎座授粉,則僅有培養在不添加生長激 素的MS培養基有12.5 %的胎座受精,每胎座僅2.7個受精胚。關鍵詞:台灣泡 桐、試管內授粉、花粉發芽。 |
英文摘要 | Pollen germination rate of P. x taiwaniana increased as flower buds elongated and bloomed but varied as sucrose concentrations changed. Pollen germinated when flower buds grew up to 30 mm long, however, at this stage sucrose was required in the BK germination medium. When flowers had bloomed thoroughly, 95% of the pollen germinated in BK medium with or without sucrose. Aseptic pollen grains from by surface-sterilized anthers of blooming flowers were used to pollinate onto in vitro placentae and stigmas of pistils. As to development stage, embryo sacs used for in vitro pistil pollination were at the 2-cell stage, while those for in vitro placenta pollination were at the 4-cell stage. For in vitro pistil pollination, 25% of placentae within pistils formed zygotic embryos in MS medium without plant growth regulators, but the greatest number of embryos, at about 100 embryos per placenta, were obtained in MS medium containing 0.5 mg/l zeatin and 0.5 mg/l IAA. In vitro placenta pollination produced much lower embryo formation when compared to in vitro pistil pollination. Only 12.5% of placentae cultured in MS medium without plant growth regulators could form 2.7 embryos. |
本系統中英文摘要資訊取自各篇刊載內容。