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題 名 | Nodule Cultures of Paulownia X Taiwaniana=臺灣泡桐的瘤狀細胞團培養 |
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作 者 | 何政坤; 陳振榮; 蔡錦瑩; 楊政川; | 書刊名 | 臺灣林業科學 |
卷 期 | 12:1 1997.03[民86.03] |
頁 次 | 頁39-45 |
分類號 | 436.1 |
關鍵詞 | 瘤狀細胞團培養; 臺灣泡桐; Nodule cultures; Paulownia x taiwaniana; |
語 文 | 英文(English) |
中文摘要 | Nodule cultures with potential applications in regeneration strategies, automated micropropagation, and in vitro phytochemical production in many plants have been reviewed. The 1st nodule cultures of Paulownia x taiwaniana were established and maintained for more than 1 y by regular subculturing.Vigorous callus clupms were induced from leaf explants cultured on 1/2MS medium containing 3 mg/L IBA and 3 mg/L BA. Nodule cultures were produced after leaf-derived calli were transferred into MS liquid medium containing 3 mg/L each of IBA and BA, and placed on a reciprocal shaker at 130 rpm for 6wk. Cell growth cycles of the 3 mo-old and 1-y-old subcultures were determined by using settled cell volume and electrical conductivity. Results obtained from these 2 measurements were consistent with each other. Conductivity appeared to be rapid, simple, and reproducible. Histological changes of cell differentiation and vascular organization were also investigated at different growth phases of cultures. Nodules maintained organogenic capability in the 1st few subcultures, but lost it in successive cultures. |
英文摘要 | 瘤狀細胞團培養在植物組織培養的再生系統、自動化的微體繁殖與植物化學物的 生產上,已被視為具有相當潛力的應用價值。本研究首次建立臺灣泡桐的瘤狀細胞團培養並 可繼代培養一年以上。 首先將葉片培植體培養在 MS 培養基添加 3mg/L IBA 與 3 mg/L BA 以誘導生長旺盛的癒合組織細胞團,再移植於 1/2 MS 液體培養基添加各 3mg/L 之 NAA 與 BA,經在 130 rpm 轉速下的迴轉震盪器培養 6 週後,瘤狀細胞團隨即被誘導出來。瘤狀細 胞團經繼代培養 3 個月及 1 年後,其生長趨勢可用細胞沈澱法及電導度法加以測定。這兩 種測定法所得的結果相當一致,但電導度法較為快速、簡單且重複性高。觀察瘤狀細胞團在 各不同生長時期組織切片,可明瞭瘤狀細胞之分化及維管束組織形成的過程。在前數個繼代 培養的瘤狀細胞團具有再生芽體的能力,但隨著繼代次數的增加其再生能力即逐漸喪失。 |
本系統中英文摘要資訊取自各篇刊載內容。