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題 名 | 溫度轉換效應對紅麴菌Monascus ruber生產二次代謝物monacolin K之影響=Effects of Temperature-shift on Monacolin K Production by Monascus Rubber |
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作 者 | 賴龍山; 陳美琪; | 書刊名 | 明志學報 |
卷 期 | 39:1 2007.06[民96.06] |
頁 次 | 頁27-38 |
分類號 | 369.36 |
關鍵詞 | 紅麴菌; 溫度瞬間轉換效應; Monascus ruber; Monacolin K; Effects of temperature-shift; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究探討菌株醱酵培養過程之溫度瞬間轉換效應對紅麴菌 Monascus ruber ATCC 18199 生產二次代謝產物 monacolin K 之影響。實驗結果顯示,若使用簡易的溫度轉換方式(即在種槽階段後之生產階段第 0天將培養溫度 28℃轉換降低至 23℃,即 28℃→23℃ at day 0),第十二天之 monacolin K之生產量可增加 55%(達153.8mg/L),此時紅麴紅色、橘色與黃色天然色素也分別增產約 17.9%、10.7%與 2.3%,但發現菌體產量卻只減少 6%;實驗觀察,此溫度轉換確會造成菌體增長型態改變、並影響醱酵液 pH變化趨勢,可能因而造成其二次代謝物的增產。 進一步在使用兩階段溫度變化,即溫度循環方法(28→23→28℃)的實驗中,本文發現在第一階段溫度轉換如預期地是會造成菌體產量的減少,而在第二階段升溫卻無法再回復此真菌之細胞成長與產物生產活性,發現monacolin K 產量僅達控制組之31%。本研究之實驗結果說明菌株之培養溫度主宰紅麴菌醱酵代謝路徑之走向,相對於研究者常用的恆溫培養,此最佳之培養溫度轉換(28℃→23℃ at day0 )確能增加紅麴菌二次代謝產物 monacolin K 之生產,此時,該產物之比生產速率的最高值可曾增高達四倍之多。 |
英文摘要 | In this study, attempts were made to investigate the effects of the shift in the culturing temperature on monacolin K production by Monascus ruber ATCC 18199. A simple temperature-shift approach ( 28℃→23℃ at day 0 of production) was proved highly valuable, where monacolin K production of day 12 was enhanced by 55% (153.8 mg/L), as compared with that of an isothermal culture at 28℃. Meanwhile, the production of Monascus-pigments was also increased by 17.9%(red), 10.7%(orange)and 2.3%(yellow), respectively. Nevertheless, biomass production was decreased by 6% only. This temperature-shift method used here closly influenced the morphology and changed the physiological state ( such as the pH kinetics), probably thus resulting in the enhancement of monacolin K production by the fungus. In the experiments using temperature-cycling (28→23→28℃), biomass prodution of the fungus decreased, as expected, in the first phase of temperature-cycling, but it was decreased further in the second phase. It was found that the fungus was unable to resume its growing activity and productivity, where monacolin K production was minimized as only 31% of that without cycling. Such experiments described that the culturing temperature served as an environmental signal for directing metabolism of M. ruber. As compared with an isothermal culture, monacolin K production by M.ruber was substantially enhanced when the optimal temperature-shift method (28℃→23℃ at day0 ) was applied in the cultures. Meanwhile the maximum of the specfic monacolin K production rate was largely increased as 4-fold of that of an isothermal culture at 28℃. |
本系統中英文摘要資訊取自各篇刊載內容。