查詢結果分析
來源資料
相關文獻
- 本土白殭菌(Beauveria bassiana)菌株之特性差異比較
- High Level Production of Polyhedra in a Scorpion Toxin-containing Recombinant Baculovirus for Better Control of Insect Pests
- 本土黑殭菌素對甜菜夜蛾幼蟲之毒效探討
- 過氧化氫在UV光照射下之裂解行為研究:氯離子及硝酸根離子之影響效應
- 關於纖維的紫外線隔離
- Color Removal of Azo Dye Wastewater by UV/H202 Reactor
- Effect of Ultraviolet-C Irradiation on Volatile Compounds of Common and High-Temperature Mushrooms
- 短波紫外線照射對洋菇和高溫菇儲藏品質之影響
- 簡介紫外線和對農作物生長的影響
- 可吸收紫外線透明包裝材料之特性、製造及應用
頁籤選單縮合
題 名 | 本土白殭菌(Beauveria bassiana)菌株之特性差異比較=Biological Characterization of Beauveria Bassiana Isolates from Taiwan |
---|---|
作 者 | 蔡勇勝; 楊芃苮; 洪德惠; 高穗生; | 書刊名 | 植物保護學會會刊 |
卷 期 | 48:2 民95.06 |
頁 次 | 頁117-128 |
分類號 | 433.3 |
關鍵詞 | 白殭菌; 甜菜夜蛾; 小菜蛾; 紫外線; Beauveria bassiana; Spodoptera exigua; Plutella xylostella; UV-B irradiation; |
語 文 | 中文(Chinese) |
中文摘要 | 本土不同白殭菌(Beauveria bassiana)菌株之菌絲生長對溫度反應頗為一致,最適溫均在24℃,次為28℃或20℃。供試菌株中以編號Bb-2菌株表現最佳,在各不同溫度處理中均以其菌落最大。在28℃下,24小時,所有菌株之孢子發芽率可達90%以上,但在20小時進行比較,菌株間之孢子發芽情形則有明顯之差異。另外,菌株產孢能力差異極大,產孢量最高與最低者差達4百餘倍,由高而低依序為Bb-1、Bb-2、Bb-3、Bb-4、Bb-5、Bb-6、Bb-7、Bb-8。菌絲產量之高低順序為Bb-2、Bb-6、Bb-7、Bb-5、Bb-3、Bb-4、Bb-8、Bb-1。在酵素活性反應試驗中,Bb-1、Bb-2、Bb-5、Bb-7、Bb-8等菌的脂肪分解?(lipase)活性明顯較Bb-3、Bb-4、Bb-6強。澱粉?(amylase)之作用則以Bb-1、Bb-2、Bb-4較佳,Bb-8最差。蛋白?(protease)活性以Bb-1、Bb-7兩菌株最高,次為Bb-6,Bb-5及Bb-8菌株再次之,最差為Bb-3。在107 conidia/ml濃度下,Bb-2、Bb-6、Bb-7、Bb-8菌株可使99%以上之供試三齡小菜蛾(Plutella xylostella)幼蟲罹病死亡,Bb-3、Bb-4、Bb-5三株菌僅分別有50.2、51.9與46.5%之幼蟲死亡率。白殭菌對四齡甜菜夜蛾(Spodoptera exigua)幼蟲之病原性極強,除Bb-7菌株所引起之幼蟲死亡率不及50%外,其餘各菌株均達90%以上。田間防治試驗結果發現Bb-1、Bb-2、Bb-8三菌株可使小菜蛾蟲數在處理一週後明顯降低。Bb-3菌株處理區小菜蛾蟲數雖未減少,但亦無明顯增加,處理組與對照組比較,均有顯著的防治效果。但平均每棵甘藍之重量,僅Bb-8菌株處理組較對照組及其他處理組重。以紫外線處理菌絲,八菌株中有Bb-1、Bb-2、Bb-3、Bb-4四菌株在經中波長紫外線(312nm,600 μw/cm2)處理8小時後仍可生長,另四菌株則完全受抑制,差異頗大。 |
英文摘要 | The optimal temperature for mycelial growth of indigenous Beauveria bassiana isolates was 24 ℃ , and suboptimal temperature was 28 ℃ or 20 ℃ . Of the 8 isolates tested, only the colony of Bb-2 isolate showed the greatest radial growth on each temperature. There was a 400-fold difference in spore production between the highest and lowest. Isolates Bb-1 exhibited the highest spore production among B . bassiana isolates tested. Rate of conidial germination was above 90% when incubated at 28 ° C, for 24 hr, but there was a significant difference among isolates tested after 20 hr incubation. In the enzyme activity tests, the results showed that isolates Bb-1, Bb-2, Bb-5, Bb-7 and Bb-8 had higher lipase activity than Bb-3, Bb-4 and Bb-6. Isolates Bb-1, Bb-2 and Bb-4 exhibited higher amylase activity than other isolates tested. Isolate Bb-8 exhibited the lowest amylase activity among B . bassiana isolates tested. In protease activity test, isolates Bb-1 and Bb-7 exhibited the highest enzymatic activity, followed by Bb-6, Bb-5 and Bb-8. Isolate Bb-3 exhibited the lowest enzymatic activity. Bioassay showed that Bb-2, Bb-6, Bb-7 and Bb-8 isolates were virulent against 3rd instar larvae of Plutella xylostella . The mortalities of P . xylostella larvae caused by these four isolates ranged from 99.1% to 99.8%. All B . bassiana isolates tested except Bb-7 were strongly pathogenic to 4th instar larvae of Spodoptera exigua . All isolates of B . bassiana tested in field trials showed high control efficacy against P . xylostella within 2 weeks. Treatment with isolate Bb-8 resulted in the highest average weight of cabbage harvested per plant among all treatments. Among eight isolates of B . bassiana tested, only isolates Bb-1, Bb-2, Bb-3 and Bb-4 exhibited colony growth on V8 plates after exposure to UV-B irradiation (312 nm, 600 μw/cm 2 ) for 8 hr. |
本系統中英文摘要資訊取自各篇刊載內容。