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題名 | High Level Production of Polyhedra in a Scorpion Toxin-containing Recombinant Baculovirus for Better Control of Insect Pests=提高多角體產量以增加具蠍神經毒表現能力之桿狀病毒的殺蟲效果 |
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作者 | 段淑人; 侯豐男; 李至芬; 趙裕展; | 書刊名 | Botanical Studies |
卷期 | 48:3 2007.07[民96.07] |
頁次 | 頁273-281 |
分類號 | 433.85 |
關鍵詞 | 加州苜蓿夜蛾核多角體病毒; 重組病毒; 以色列黃蠍; 鎮定神經毒; 甜菜夜蛾; 擬尺蠖; 小菜蛾; 多角體量產; 防治效果; Autographa californica nucleopolyhedrovirus; AcMNPV; Leiurus quinquestriatus hebraeus; Insecticidal efficacy; Neurotoxin; Plutella xylostella; Polyhedral production; Recombinant virus; Spodoptera exigua; Trichoplusia ni; |
語文 | 英文(English) |
中文摘要 | 含蠍毒桿狀病毒之多角體量產的成功與否,是成為害蟲防治利器的關鍵因素。本研究中兩種由加州苜蓿夜蛾核多角體病毒(Autographa californica (Speyer) nucleopolyhedroviruses, AcMNPVs)改造而成含以色列黃蠍之鎮定神經毒(Leiurus quinquestriatus hebraeus (Ehrenberg), LqhI□)之重組病毒vAPcmI□及vAP10I□ (Tuan et al., 2005),分別利用早期起動子(p-PCm)及及非常晚起動子(p10)來表現蠍毒基因。試驗目在於探討兩種重組病毒多角體的量產效率,發現無論係在體內或體外系統,早期表現蠍之重組病毒均顯著優唹晚期表現蠍毒之重組病毒。以Sf21細胞株生產病毒,vAPcmI□的量產效果較vAP10I□顯著良好,以擬尺蠖(Trichoplusia ni (Hubner))及甜菜夜蛾(Spodoptera exigua (Fabricius) )兩種幼蟲繁殖病毒時,發現雖然vAP10I□的產量尚不如野生型AcMNPV,但卻是vAP10I□多角體產量的十倍。含蠍毒重組病毒對兩種具抗藥性的蔬菜害蟲-甜菜夜蛾及小菜蛾(Plutella xylostella (Linnaeus))幼蟲之起始致死時間(L□)及半致死時間(L□)均較野生型病毒有顯著提早之效果。在甘監盆栽試驗,含蠍毒重組病毒導致甜菜夜蛾幼蟲提早死亡,表現90%以上的防治效果,且較野生型病毒處理顯著減少58.7~67.4%之葉片受害率。以病毒多角體量產的效率及防治效果而言,早期表現蠍毒之重組病毒vAPcmI□較晚期表現蠍毒之重組病毒vAP10I□及野生型病毒更具開發成生物農業的優勢。 |
英文摘要 | A sufficient occlusion body yield by a scorpion toxin-containing baculovirus is important to the success of a pest management program. In this study, recombinant baculoviruses, AcMNPV, vAPcmI□, by which the scorpion toxin (LqhiT□) is driven by an early phase promoter (p-PCm); and another recombinant baculovirus vAP101□, by which LqhI□ is driven by a very late p10 promoter, were tested for the efficiency of their polyhedral production. In Sf21 cells, he yield of polyhedra by vAPcmI□ is significantly better than that of vAP10I□. Tlthough in Trichoplusia ni (Hubner) and Spodoptera exigua (Fabricius) the polyhedra yields by vAPcmI□-infected larvae were not as good as those of the wild type virus, they were about tenfold higher than those produced by vAP10I□-infected larvae. To test the insecticidal activity of these recombinant baculoviruses, vAPcmI□ and vAP10I□ were applied against two majorpesticide-resistant vegetable pests, Plutella xylostella (Linnaeus) and S. exigua. Our results demonstrated a significant shortening of the lethal time (L□ and L□) compared to those larvae infected with wild type AcMNPV. In field trials, larvae of S. exigua infected with the toxin-recombinant viruses provided more than 90% control efficacy and resulted in a 58.7~67.4% reduction in leaf area consumed compared to wild type AcMNPV. Based on the efficacy of polyhedral production and crop protection, the superiority of vAPcmI□ over both vAP10□ and wild type AcMNP renders it a better candidate to serve as a useful biopesticide. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。