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頁籤選單縮合
題名 | Detection of Lactoferrin in Bovine and Goat Milk by Enzyme-linked Immunosorbent Assay=利用酵素連結免疫分析法檢測牛羊乳中的乳鐵蛋白濃度 |
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作者姓名(中文) | 陳柏文; 毛嘉洪; | 書刊名 | 藥物食品分析 |
卷期 | 12:2 2004.06[民93.06] |
頁次 | 頁133-139+195 |
分類號 | 412.37 |
關鍵詞 | 酵素連結免疫分析法; 牛乳; 羊乳; 乳鐵蛋白; Lactoferrin; Milk; ELISA; |
語文 | 英文(English) |
中文摘要 | 乳鐵蛋白為食品中具有多功能之機能性成份。為了檢測乳中的乳鐵蛋白含量,本研究利用牛乳鐵蛋白的抗血清及牛乳鐵蛋白與生物素的聯結體,建立競爭型的酵素連結免疫分析法,並以此法檢測牛乳中的乳鐵蛋白濃度。然而,此方法無法檢測羊乳中的乳鐵蛋白含量,因此使用羊乳鐵蛋白與生物素聯結體以建立羊乳鐵蛋白酵素連結免疫分析法,而此法可成功地檢測羊乳中的乳鐵蛋白濃度。一些乳樣本中的乳鐵蛋白濃度也隨之被檢測。結果指出,當個別牛乳中體細胞數目小於每毫升十萬以下、十萬至二十五萬或二十五萬至五十萬時,其乳中的乳鐵蛋白濃度平均值分別為每毫升176、466或742微克。當總羊乳之美藍還原試驗時間超過五小時,其乳中的乳鐵蛋白平均濃度約為200微克。總之,本研究成功地建立了兩種酵素連結免疫分析法而能檢測牛羊乳中的乳鐵蛋白濃度。 |
英文摘要 | Lactoferrin (LF) has been proposed as a multifaceted functional ingredient of food. A competitive enzyme-linked immunosorbent assay (ELISA) was established to determine the LF in milk by using bovine LF antiserum and bovine LF-Biotin conjugate. The LF concentration in cow milk samples could be determined by this ELISA. However, the LF concentration in goat milk could not be measured using this method. Thus, the goat LF ELISA was established using goat LF-Biotin conjugate to measure the LF concentration in goat milk. Milk samples were collected for measurement of their LF concentrations. The mean LF level for the individual cow milk samples with the somatic cell count (SCC) below 1 x 105, 1 to 2.5 x 105 or 2.5 x 105 to 5 x 105 cells/mL was found to be about 176, 466 or 742 mg/mL, respectively. On the other hand, the LF level was about 200 mg/mL in goat bulk milks with the methylene blue reduction test (MBRT) time more than 5 hr. In conclusion, both LF ELISA methods developed in this study could be successfully used to determine the LF concentration in goat and cow milk. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。