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題 名 | 細胞內抗壞血酸抑制β-類胡蘿蔔素促氧化作用=Inhibition of Pro-oxidative Activity of β-Carotene in Human Cells by Intracellular Ascorbic Acid |
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作 者 | 葉姝蘭; 吳家淑; 胡淼琳; | 書刊名 | 中華民國營養學會雜誌 |
卷 期 | 28:4 2003.12[民92.12] |
頁 次 | 頁226-233 |
分類號 | 411.38 |
關鍵詞 | 抗壞血酸; 去氫抗壞血酸; β-胡蘿蔔素; 交互作用; Ascorbic acid; Dehydroascorbic acid; β-carotene; Interaction; |
語 文 | 中文(Chinese) |
中文摘要 | 有關抗壞血酸(ascorbic acid, AA)是否會與β-胡蘿蔔素(β-carotene, BC)直接作用而可抑制後者的自氧化及促氧化性是一個懸而未決的問題。我們最近發現:BC會促進脂溶性自由基發生劑2,2’-azobis(2, 4-dimethylvaleronitrile)(AMVN)對人類上皮層纖維母細胞(Hs68)之氧化傷害,因此本研究利用Hs68及人類胚胎腸道細胞(Int407)分別以AA或去氫抗壞血酸(dehydroascorbate ,DHA)預培養24小時,再以20μM BC培養1小時後,加50mM AMVN處理2-3小時的模式來探討細胞內AA與BC的交互作用。測定項目包括脂質過氧化、DNA傷害以及細胞中AA和BC的損耗情形。結果顯示:單獨以20μM AA 或DHA預培養後,在兩株細胞中均可顯著減少AMVN所誘發之DNA傷害,但僅在Int407中對降低脂質過氧化有顯著效果。20μM AA或DHA預培養之細胞若再經BC培養並經AMVN處理後,BC對細胞促氧化現象顯著降低,對脂質過氧化抑制尤其明顯。AA與BC在AMVN處理下的消耗分析中,AA僅能部分防止BC的消耗,且BC的消耗速率並不因AA 含量不同而不同。以上結果顯示:細胞內AA 直接將細胞膜上BC自由基還原回BC之可能性相當低,AA可能藉由阻止BC氧化產物繼續反應而減少BC消耗,進而降低其促氧化性。 |
英文摘要 | Inconsistent results exist in the literature regarding whether ascorbic acid (AA) interacts with β-carotene (BC) directly and decreases BC autooxidation and pro-oxidative activity. We previously reported that BC enhances oxidative damage in human foreskin fibroblasts (Hs68) induced by 2,2’-azobis (2,4-dimethyl-valeronitrile) (AMVN). Herein, we investigated whether intercellular AA reduces the pro-oxidative activity of BC induced by AMVN in Hs68 cells and human embryo intestine cells (Int407) and whether such an effect is direct or indirect. Both types of cells were pre-incubated with AA of dehydroascorbate (DHA) with or without subsequent incubation with 20 μM BC, followed by 50 mM AMVN treatment. Lipid peroxidation, comet assay and the consumption of intercellular AA and BC were determined. The results showed that incubation of both cell types with 20μM AA or DHA significantly decreased AMVN-induced DNA damage, but the effect of such a treatment on lipid peroxidation was significant only in Int407 cells. By contrast, lipid peroxidation induced by AMVN was significantly decreased in both cell lines pre-incubated with either AA or DNA with subsequent incubation with BC. However, intracellular AA only slightly decreased the consumption of BC by AMVN and this effect of AA was not concentration-dependent, indicating the impossibility of direct recycling of BC by AA. The results suggest that protection against the pro-oxidative activity of BC by AA in cells is indirect, possibly through blocking the chain reaction of BC oxidative products. |
本系統中英文摘要資訊取自各篇刊載內容。