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題名 | Monodehydroascorbate Reductase cDNA from Sweet Potato: Expression and Kinetic Studies=單去氫抗壞酸還原酶在大腸桿菌中的表現及其生化特性研究 |
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作者 | 黃智郁; 溫麗莎; 莊榮輝; 許垤棊; 林棋財; | 書刊名 | Botanical Studies |
卷期 | 51:1 2010.01[民99.01] |
頁次 | 頁37-44 |
分類號 | 434.313 |
關鍵詞 | 甘藷; 相似性立體結構; 基因表現; 單去氫抗壞血酸還原酶; Ipomoea batatas [L.] Lam; 3-D homology structure; Monodehydroascorbate reductase, MDAR; Sweet potato; 3-D homology structure; Expression; Monodehydroascorbate; MDAR; |
語文 | 英文(English) |
中文摘要 | 單去氫抗壞血酸還原酶 (monodehydroascorbate reductase, MDAR) 在抗氧化中還原單去氫抗壞血酸 (MDA) 成抗壞血酸 (AsA) 。依據甘藷 (Ipomoea batatas [L.] Lam) 表現庫序列資訊 (expressed sequence tag database) 選殖出MDAR cDNA 序列 (1,548 bp, EMBL accession no. EF118034),全長共1,548 個核苷酸,內含轉譯區1302 個核苷酸,可轉譯出434 個胺基酸的還原酶。經序列比較IbMDAR 與其他物種的序列有很高的相似性,依據已知結構,建立一相似性立體結構 (3-D homology structure) 。進一步將其轉譯區選殖入表現載體 pET-20b(+) ,以大腸桿菌 E. coli BL21(DE3)pLysS 作為表現宿主,經親和性管柱純化可得到具有活性的IbMDAR ,其特性在70°C 加熱活性降低一半的時間為12.4 分鐘,在pH 6.0 ~ 10.0 仍然具有相當的活性。對 MDA 和NADH 其Km 值分別為21.1 和 39.7 μM。 |
英文摘要 | A cDNA encoding a putative monodehydroascorbate reductase (MDAR) was cloned from sweet potato. The deduced protein showed high level of sequence homology with MDARs from other plants or related family from bacteria (23~80%). A 3-D homology structure was created for this MDAR. Functional sweet potato MDAR was expressed and purified. The purified enzyme showed an active monomeric form on a 10% native PAGE. The protein’s half-life of deactivation at 70°C was 12.4 min, and its thermal inactivation rate constant Kd was 5.6 × 10-2 min-1. The enzyme was stable in a broad pH range from 6.0-10.0, and in the presence of 0.8 M imidazole. The K m value for monodehydroascorbate (MDA) and NADH were 21.1 and 39.7 μM, respectively. |
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