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題名 | 綬草無菌播種與生長發育=In vitro Seed Germination and Plant Development of Spiranthes sinensis (Pers.) Ames |
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作者 | 張正; 陳盈君; 嚴新富; | 書刊名 | 中國園藝 |
卷期 | 49:4 2003.12[民92.12] |
頁次 | 頁407-410 |
分類號 | 435.431 |
關鍵詞 | 綬草; 種子發芽; 生長發育; 開花; Spiranthes sinensis (Pers.) Ames; Seed germination; Development; Flowering; |
語文 | 中文(Chinese) |
中文摘要 | 授草(Spiranthes sinensis(Pers.)Ames)蒴果消毒後,將種子播在添加20 g/l蔗糖、50 g/l香蕉泥、1 g/l活性碳的1/4 MS基礎培養基,一個月後,形成原球體。培養五個月後,原球體長成葉長約2公分、根2-3條的幼苗,但其中有13.4%為白化苗。將綠葉幼苗繼代到添加20 g/l蔗糖、30 g/l馬鈴薯泥、1 g/l活性碳、150 ml/l椰子水的1/4 MS基礎培養基,再經二個半月後,幼苗可長到5公分長。幼苗經馴化後移植出瓶,97%的小苗可存活。且在四個月後,65%小苗抽苔開花,花色如同母株為粉紅色,但有2.5%為白花植株。此法可在一年之內完成授草從種子發芽到成苗開花的生活史。 |
英文摘要 | The capsules of Spiranthes sinensis (Pers.) Ames. were sterilized by diluted sodium hypochlorite solution and the seeds were sown in the medium containing 1/4 strength of MS basal medium supplemented with 20 g/l sucrose, 50 g/s banana pulp and 1 g/l activated charcoal. Green protocorms were then observed after one month. The seedlings were grown to plantlets with shoot and root after 5 months of culture. The plantlets were then subcultured onto the medium containing 1/4 strength of MS salt basal medium, 20 g/l sucrose, 30 g/l potato pulp, 1 g/l activated charcoal and 150 ml/l coconut milk. After 75 days of culture, the seedling (about 5 cm in height) were transplanted into a greenhouse with 97% survival rate. After 4 months of growing, 65% acclimatized plantlets flowered. The complete procedure of propagation the spiranthes orchids took less one year from seed germination to flowering. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。