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題名 | 龍蝦殼製備之幾丁聚醣圓珠與香菇提鍊之酪胺酸酵素固定化--酵素催化反應生產L-DOPA之研究=Preparation of Chitosan Beads from Lobster Shell and Extraction of Tyrosinase form Lentinula Edodes: The Study of Production of L-DOPA by the Catalytic Reaction of Immobilized Enzyme |
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作者 | 賀邦彥; 吳豐智; Ho, Pang-yen; Wu, Feng-chin; |
期刊 | 聯合學報 |
出版日期 | 20010500 |
卷期 | 18 2001.05[民90.05] |
頁次 | 頁145-152 |
分類號 | 460.025 |
語文 | chi |
關鍵詞 | 龍蝦殼; 幾丁聚醣圓珠; 香菇之酪胺酸酵素; 酵素; 催化反應; Chitosan bead; Lobster shell; Enzyme immobilization; Tyrosinase; L-DOPA; |
中文摘要 | L-DOPA 為治療帕金森氏症最有效藥物之一,本研究利用南投縣魚池鄉收購之新鮮 香菇,製成丙酮粉,並萃取出酪胺酸酵素;以龍蝦殼去除蛋白質和礦物質,並經去乙醯化反 應獲得之幾丁聚醣,再經溶解及成型為幾丁聚醣圓珠。幾丁聚醣圓珠與戊二醛交聯反應後, 再與酪胺酸酵素溶液固定化; 含 L- 酪胺酸和維他命 C 酸之反應液與固定化圓珠進行酵素 催化反應,生成 L-DOPA,其中探討戊二醛之交聯, 丙酮粉萃取之緩衝液,酪胺酸酵素固定 化和酵素催化反應之各種變數等,綜合獲得最佳操作條件,可加速 L-DOPA 生成率 3.84 倍 ,分別在 8 和 120 小時,生成 L-DOPA 為 57.2 和 176mg/L;判斷反應系統若具回流功能 ,則以反應 8 小時為佳,若為單一批式系統,則在 120 小時反應可得高生成量。 |
英文摘要 | The production of L-3,4 dihydroxyphenylalanine (L-DOPA) for tyrosine by the catalytic reaction of immobilized tyrosinase on chitosan beads was investigated in a batch reactor. Tyrosinase was extracted from lentinula edodes and the chitosan beads were prepared from lentinula edodes and the chitosan beads were prepared from lobster shell. The effects of glutaraldehyde concentration, pH value, reaction temperature, and the reaction time upon the production rates of L-DOPA were discussed. In order to obtain a higher production rate of L-DOPA, the optimal condition of immobilized enzyme wad 10%(W/V) glutaraldehyde in sodium phosphate solution (0.2M, pH6.0). It was helpful for immobilized enzyme reacted with tyrosine in a acidic solution (even the pH value was lowered to 3), while ascorbic acid was used as a reducing agent to facilitate the production of L-DOPA. A series of experiments were investigated for the production of L-DOPA. The optimal temperature for this enzymatic reaction was 40 ℃ with a production rate of 3.84 fold compared with operated at 25 ℃. Under the same operating conditions for the immobilization and catalytic reaction, the L-DOPA produced was approximately 57.2mg within 8 hours and 176mg within 120 hours in one liter, respectively. |
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