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題 名 | 電子鼻感測元件分子識別材料之探索研究=Exploring the Recognized Molecules as Sensing Components for Use in "Electronic Nose" |
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作 者 | 吳宗正; 羅淵仁; 劉世隆; 林于生; | 書刊名 | 化學 |
卷 期 | 59:2 2001.06[民90.06] |
頁 次 | 頁235-243 |
專 輯 | 化學感測器專題 |
分類號 | 342.87 |
關鍵詞 | 電子鼻; 分子識別材料; 合成胜冴; 嗅受體蛋白質; 分子模擬; 氣體感測探針; Electronic nose; Recognized molecules; Synthetic peptide; Olfactory receptor; Molecular simulation; Gas sensing probe; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究先建立狗的嗅受體蛋白和三甲基胺(trimethylamine)氣體的三級結構,再 利用分子模擬與動力學計算來尋找兩者間最可能的親合位置。然後根據親合位置附近的胺基 酸序列資訊,設計出對該目標氣體具感測能力的寡胜�顜レC (orp61 和 orp188),進而予以 合成及純化,接著塗覆在壓電晶體的金電極表面製成氣體感測電極。經由對目標氣體的測試 ,這兩段序列對三甲基胺具有很好的選擇性吸附能力。為了更進一步加強胜�韝壑l對三甲基 胺的特異性吸附能力,另藉由胜�顜レC定位修飾法 (site-specific modification) 來改變 親合位之極性與契合程度。研究結果顯示,orp61 序列經過修飾其中兩個胺基酸後,對目標 氣體 -- 三甲基胺的靈敏度增加了約 33 %,且對其他干擾氣體呈降低現象。此技術除提供 一個獨特的分子識別材料取得之研究方向外,更對未來有關氣體偵測所需之分子識別材料的 研究建立學術基礎,尤其在電子鼻技術的開發領域上。 |
英文摘要 | A putative tertiary structure model of the dog's olfactory receptor (olfd canfa) is established in this study, By using a target odorous compound (trimethylamine), it is possible to locate the most plausible binding sites between the receptor model structure and the target odorous molecules through computer docking simulations. The two short oligo-peptide sequences (orp61 and orp188) for trimethylamine sensing were identified, synthesized, purified and ocated onto the surface of the separate piezoelectric gold electrodes. These two peptides show a high binding capability for trimethylamine. To further enhance the sensitivity of the polypeptides towards the target compound, the polarity and the degree of docking were changed by a site-specific modification technique. The orp61 sequence was modified by substituting two amino acids in the binding picket resulting in 33% incrase in sensitivity towards trimethylamine and reduced noises from other non-target chemicals. The techniques used in the present study offer a unique approach for synthesizing peptides in mimicking binding domain of olfactory receptors. The approach can be easily applied to further development of recognized molecules for gas sensing, especially for use in `electronic noses'. |
本系統中英文摘要資訊取自各篇刊載內容。