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題名 | Effect of Ammonium Concentration on the Cultivation of Recombinant Escherichia Coli Producing Penicillin G Acylase=培養基中銨濃度對基因改質大腸桿菌醱酵生產盤尼西林醯胺酵素之影響 |
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作者 | 劉永銓; 陳光宇; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 31:6 2000.11[民89.11] |
頁次 | 頁601-607 |
分類號 | 460.02 |
關鍵詞 | 銨濃度; 基因改質; 大腸桿菌; 醱酵; 盤尼西林醯胺酵素; Penicillin G acylase; E. coli; Ammonium; Metabolic pathway; |
語文 | 英文(English) |
中文摘要 | 本研究以-經基因改變帶有pGL-5質體之大腸桿菌,醱酵生產盤尼西林醯胺酵素(PGA),並探討不同銨濃度對醱酵造成之影響。在培養基中三種不同硫酸銨濃度分別為0.5%,3%,5%,結果顯示在這些不同硫酸銨濃度下,醯酵全程中均僅有小量醋酸、乳酸及丙酮酸副產物產生,然而在3%硫酸銨時,有較多之乳酸之累積。而在5%硫酸銨時,於醱酵10小時後開始有大量丙酮酸之累積。培養基中硫酸銨濃度愈高,菌體生長之遲滯期愈長。在3%及5%硫酸銨之培養時,菌體生長速率均為後對數生長期大於前-中對數生長期。在5%硫酸銨下,菌體生長及PGA之生產明顯受到抑制。 |
英文摘要 | The effect of ammonium on the cultivation of r E. coli UP1, a penicillin G acylase (PGA) hyper-producer, was investigatd. While r E. coli UP1 was grown under three different concentrations of ammonium, i.e., 0.5%, 3% and 5% ammonium sulfate, respectively, it was found that the higher the ammonium sulfate concentration, the longer the lag phase. Cell growth and PGA activity expression were not inhibited in the 0.5-3% ammonium sulfate cases, whereas significant regulation of cell growth was found in the 5% ammonium sulfate case. The time profile of organic acids was affected by the ammonium sulfate concentration in the medium. In the 3% ammonium case, a higher amount of lactic acid was observed. In contrast, traces of pyruvic acid were detected during fermentation expect for the case with 5% ammonium sulfate, where pyruvic acid accumulated significantly from 10 h of fermentation. |
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