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頁籤選單縮合
題名 | VP2抗原基因選殖在大腸桿菌醱酵的探討 |
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作 者 | 張玲玲; 黃麗月; | 書刊名 | 華岡工程學報 |
卷期 | 7 1994.05[民83.05] |
頁次 | 頁1-10 |
分類號 | 364.6 |
關鍵詞 | VP2抗原基因; 大腸桿菌; 選殖; 醱酵; |
語文 | 中文(Chinese) |
中文摘要 | 重組大腸桿茵用T7 promoter啟動豬病疫苗VP2基因製造,T7 promoter有個lac operon所以必須加入inducer IPTG才能啟動VP2基因。但是IPTG價位太高若想量產豬病疫 苗不合乎經濟成本,所以本文探討用IPTG analogue lactose取代IPTG的可行性,發現適當 控制醱酵條件,經過10-12小時醱酵,加入乳糖取代葡萄糖,再經過4小時,菌體度達20D600 , VP2表現度佔全部蛋白質的15%-20%。 |
英文摘要 | Recombinant E. coli used T7 promoter to produce VP2 gene for porcine vaccine. It is necessary to add inducer IPTG (isopropythio galactoside) to produce VP2gene because there is a lac operon in the T7 promoter. If we want to mass production VP2gene, we have to spend much money in inducer IPTG. In this research, we want to study the feasibility of replacing high cost of IPTG by low cost of lactose, The result is that it takes 10-12 hours fermentation then to add lactose. The lactose one is as a carbon source the other is as an inducer. The cell density and VP2 gene expression achieve 20 OD600 and 15-20% respectively after adding lactose 3-4 house. |
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