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題 名 | Effect of Carbon Source on Inclusion Body Formation upon Overproduction of Periplasmic Penicillin Acylase in Escherichia coli=碳源對於大腸桿菌中大量生產Penicillin Acylase之影響 |
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作 者 | 周志雄; 曾仁宏; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷 期 | 31:3 2000.05[民89.05] |
頁 次 | 頁219-224 |
分類號 | 460.02 |
關鍵詞 | 碳源; 大腸桿菌; 糖類添加; 包涵體; Penicillin acylase; Inclusion body; Gene expression; |
語 文 | 英文(English) |
中文摘要 | 本文主要探討不同糖類添加對Penicillin Acylase之生產與包涵體形成之效應。 |
英文摘要 | The effect of carbon supplementation on penicillin acylase (PAC) production and inclusion body formation was investigated. The host effects was also examined by comparing culture performances using HB101 or its derived mutant, MD□P7, as the expression host. Among several carbons tested, glycerol was found to be optimum for PAC production. PAC activity was increased and the amount of inclusion bodies was reduced upon glycerol supplementation. The effect of carbon source on inclusion body formation appears to be strain-dependent. With the optimization on the culture parameters (i.e., host and carbon effects), volumetric PAC activity could be significantly improved at more than fourfold that of the control experiment. This information is useful for developing expression strategies for optimization of PAC production. |
本系統中英文摘要資訊取自各篇刊載內容。