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題 名 | Use of Polymerase Chain Reaction to Detect Proteus Mirabilis and Ureaplasma Urealyticum in Urinary Calculi=使用聚合酵素連鎖反應來偵測尿石中的變形桿菌和尿漿菌 |
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作 者 | 黃鶴翔; 陳淳; 鄧麗珍; 賴明坤; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 98:12 1999.12[民88.12] |
頁 次 | 頁844-850 |
分類號 | 415.842 |
關鍵詞 | 聚合酵素連鎖反應; 尿石; 變形桿菌; 尿漿菌; Urinary calculi; Polymerase chain reaction; Proteus mirabilis; Ureaplasma urealyticum; Urinary tract infection; |
語 文 | 英文(English) |
英文摘要 | In this study, we evaluated the efficacy of the polymerase chain reaction (PCR) in detecting urea-splitting microorganisms in desiccated urinary tract infection stones. Seventy-eight urinary tract stones were tested for the presence of Proteus mirabilis and Ureaplasma urealyticum by means of PCR with species-specific primers. Twenty-seven stone samples were composed of struvite and/or carbonate apatite (infection stone); 40 were calcium oxalate and/or calcium phosphate; seven were mixed, with struvite/carbonate apatite and calcium oxalate; and four were uric acid stones. PCR was performed with DNA extracted from pulverized stone pieces. Initial assays using the pulverized stone specimens spiked with microorganisms showed that PCR could not detect U. urealyticum at densities below 10 �� color changing units (CCU), or P. mirabilis at densities below 10 �� colony-forming units (CFU). PCR was negative for U. urealyticum and P. mirabilis in all metabolic stones from patients. P. mirabilis was detected by PCR in 10 of 34 patients with infection stones. Preoperative urine cultures grew P. mirabilis in three of these 10 patients, and were negative for P. mirabilis in the other seven. U. urealyticum was detected by PCR in stone samples from four patients, two of which were also PCR-positive for P. mirabilis. All four of these patients had infection stones; two had residual stones, and the other two had recurrence of urinary stones after their operations. These results demonstrate that microorganisms in urinary stones can be detected by PCR even when the voided urine culture is negative. Investigations into the role of bacterial infection in stone formation will require further improvments in the sensitivity of PCR assays for pathogen detection. |
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