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頁籤選單縮合
題名 | 利用PCR選殖落花生rDNA之IGS區域=Cloning of Intergenic Spacer Region of rDNA in Peanut by Polymerase Chain Reaction |
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作者 | 蔡奇助; 黃勝忠; | 書刊名 | 臺中區農業改良場研究彙報 |
卷期 | 61 1998.12[民87.12] |
頁次 | 頁1-11 |
分類號 | 434.255 |
關鍵詞 | 聚合酵素連鎖反應; 核糖體DNA; 基因間隔區; 落花生; 選殖; Polymerase chain reaction; Ribosomal DNA; Intergenic spacer; Peanut; Cloning; |
語文 | 中文(Chinese) |
中文摘要 | 本研究設計一組引子 (primer),序列分別為 IG1: 5’ TTGCTGCCACGATCCACTGAG 3’和 IG2: 5’ CTACTGGCAGGATCAACCAGG 3’。此引子組經聚合酵素連鎖反應 (polymerase chain reaction, PCR),可將各品種的落花生之核糖體 DNA (rDNA) 基因間隔區 (intergenic spacer, IGS)有效選殖 (cloning) 出來。經電泳分離,其PCR 產物長約 1,650 bp。將其中一個 PCR產物進行部分定序 (sequencing),以進一步確認 PCR所選殖出的 DNA片段確為rDNA的IGS區域。定序結果與其它植物之相對應區域進行序列比對,証實我們所設計的引子組確能將落花生 rDNA的IGS區域選殖出來。 |
英文摘要 | One set of primers, IG1: 5’ TTGCTGCCACGATCCACTGAG 3’ and IG2: 5’ CTACTGGCAGGATCAACCAGG 3’, was designed for cloning the intergenic spacer (IGS) of ribosomal DNA (rDNA) in peanut by using polymerase chain reaction (PCR). The primers could efficiently amplify the IGS of rDNA in peanut. After separating by agarose gel, the length of PCR products was approximately 1,650 bp. The PCR products were then sequencing analyzed and compared with IGS regions of other plant species. The result suggests that it is the IGS region of peanut. This paper present that the PCR techniques could be applied to clone the IGS of rDNA from peanut with a specific set of designed primers. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。