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頁籤選單縮合
題名 | Investigation of an Outbreak of Pseudomonas Putida Using Antimicrobial Susceptibility Patterns, Pulsed-Field Gel Electrophoresis of Genomic DNA and Restriction Fragment Length Polymorphism of PCR-Amplified rRNA Operons=利用抗微生物劑感受性、脈衝式電泳及核醣體核酸基因切割型對Pseudomonas putida之群突發調查 |
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作 者 | 翁麗涓; 廖國楨; 王乃玉; 王淑芬; 李聰明; 黃富源; 楊定一; 江春雪; | 書刊名 | 微免與感染雜誌 |
卷期 | 32:3 1999.09[民88.09] |
頁次 | 頁187-193 |
分類號 | 414.8 |
關鍵詞 | 抗微生物劑感受性; 脈衝式電泳; 核醣體核酸基因切割型; Pseudomonas putida; Antimicrobial susceptibility; Pulsed-field gel electrophoresis; PFGE; Restriction fragment length polymorphism; RFLP; rRNA operons; |
語文 | 英文(English) |
中文摘要 | 以抗微生物劑感受性(antimicrobial susceptibility)、脈衝式電泳 ( pulsed-field gel electrophoresis (PFGE) of genomic DNA )及核醣體核酸基因切割 型( restriction fragment length polymorphism (RFLP) of PCR-amplified rRNA operons )三種方法, 對來自 14 個病人的尿液檢體以及 1 個尿比重計之 17 株 Pseudomonas putida 作菌株分型,以判斷是否有群突發感染的發生。 抗微生物劑感受性將 此 17 株菌株分為三型,其中 14 株菌株對測試之 22 種抗微生物劑皆具有抗藥性。脈衝式 電泳將此 17 株菌株分為 9 型,如果將含有最多菌株( 6 株)的一型當作母型,則其他 8 型可分別為與母型相近的 4 型( 6 株),與母型可能相近的 2 型( 2 株),以及與母型 不同的 2 型( 3 株)。 屬於母型及與母型相近的 4 型,總共 12 株菌株,皆來自開刀加 護病房及開刀病房,與來自其他病房的菌株不同,明顯地表示此 12 株菌株可能是由於群突 發感染。核醣體核酸基因切割型可將此 17 株菌株分別為 2 型,其中一型僅有 2 株,此 2 株恰好亦屬於與母型不同的脈衝式電泳型。 由此研究得知,對 P. putida 的菌株分析而言 ,脈衝式電泳比抗微生物劑感受性或是核醣體核酸基因切割型較為敏感與適用。 |
英文摘要 | Seventeen Pseudomonas putida isolates were investigated which were collected from the urine specimens of 14 patients and one reflectrometer by comparing antimicrobial susceptibility patterns, pulsed-field gel electrophoresis (PFGE) of genomic DNA, and restriction fragment length polymorphism (RFLP) of PCR-amplified rRNA operons. Three susceptibility patterns were defined by testing 22 antimicrobial agents, with 14 isolates resistant to all agents. PFGE of XbaI-genomic DNA fragments divided the 17 isolates into 9 distinct types. One type, seen in 6 isolates showing identical patterns of approximately 35 fragments of 10 to 350 kb, was defined as the outbreak strain. Another 4 types, in a total of 6 isolates, were considered closely related to the outbreak strain; 2 types in 1 isolate each were possibly related to the outbreak strain; and 2 types in a total of 3 isolates were different from the outbreak strain. All 12 outbreak or closely related isolates were from patients in the surgical intensive care unit and a surgical ward, and were different from isolates in other wards, clearly indicating an outbreak of P. putida. Only two types were defined by the RFLP of 4.5 kb PCR-amplified rRNA operons; one type was seen in 15 isolates, while the other was seen in only 2 isolates. In conclusion, PFGE of genomic DNA is a highly discriminatory and reproducible method for epidemiological typing of P. putida. |
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