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題 名 | DNA Polymorphism of Mycobacterium Abscessus Analyzed by Infrequent-Restriction-Site Polymerase Chain Reaction=利用低頻切位限制酵素鏈鎖反應分析Mycobacterium Aabscessus的DNA多型性 |
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作 者 | 蘇玲慧; 賈儒馨; 呂學重; 鄭書瑋; 郭安靜; 孫建峰; 吳竹蘭; | 書刊名 | 長庚醫學 |
卷 期 | 23:8 2000.08[民89.08] |
頁 次 | 頁467-475 |
分類號 | 417.133 |
關鍵詞 | 脈衝式電泳分析; 低頻切位限制酵素鏈鎖反應; 分子生物分型; Mycobacterium abscessus; Pulsed-field gel electrophoresis; PFGE; Infrequent-restriction-site polymerase chain reaction; IRS-PCR; Molecular typing; |
語 文 | 英文(English) |
英文摘要 | BACKGROUND: Mycobacterium abscessus is an important pathogen that has been increasingly associated with many clinical and nosocomial infections. A reliable molecular typing scheme is essential for the epidemiological study of this rapidly growing mycobacterium. Pulsed-field gel electrophoresis (PFGE), considered to be the gold standard among molecular typing methods, has failed to provide satisfactory results in the molecular typing of this bacterium. A newly developed molecular typing method, infrequent-restriction-site polymerase chain reaction (IRS-PCR), was examined in this study to determine its suitability for fingerprinting M. abscessus isolates. METHODS: Eight clinical isolates of M. abscessus and two reference strains (M. abscessus ATCC 19977 and M. chelonae ATCC 35749) were studied by DNA macrorestriction analysis with XbaI resolved by PFGE, and IRS-PCR assay with adaptors designed for XbaI and HhaI restriction sites. RESULTS: By PFGE, different banding patterns were found in two clinical isolates of M. abscessus; the other isolates yielded only broken DNA and could not be assessed. By IRS-PCR, unique patterns were noted for the 10 isolates; the 10 appeared to be genetically different. CONCLUSION: IRS-PCR may be an efficient substitute for PFGE in analyzing the DNA polymorphism and epidemiology of M. abscessus. |
本系統中英文摘要資訊取自各篇刊載內容。