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題名 | 洋桔梗上胡瓜嵌紋病毒之分離及鑑定=Purification and Characterization of a Cucumovirus from Lisianthus Rusellianus |
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作者 | 陳慶忠; 胡仲祺; Chen, C. C.; Hu, C. C.; |
期刊 | 植物保護學會會刊 |
出版日期 | 19990900 |
卷期 | 41:3 1999.09[民88.09] |
頁次 | 頁179-198 |
分類號 | 435.46 |
語文 | chi |
關鍵詞 | 洋桔梗; 胡瓜嵌紋病毒; 分離; 鑑定; 蚜蟲; 傳播; Lisianthus rusellianus; Cucumber mosaic virus; Purification; Characterization; Aphid; Transmission; |
中文摘要 | 1994年南投市田間種植之洋桔梗發生葉片黃化、嵌紋及植株矮化等徵狀。罹病葉粗汁液陰染後在 電子顯微鏡下可檢視出直徑約28 nm之球形病毒顆粒。以其粗汁液機械接種15科53種植物結果12科 27種受到感染,其中20種呈系統性感染,7種呈局部感染,26種為非寄主植物。新分離之病毒可感 染多種瓜類作物,但不能感染菜豆及豇豆。病毒外鞘蛋白經SDS-PAGE分析顯示其分子量與CMV之 鞘蛋白同為29 kDa。血清學技術包括酵素聯結免疫分析、雙向免疫擴散及西方漬染法均顯示洋桔梗 上之病毒與CMV-NT9有不可區分之血清類緣關係。病毒所含四種不同分子量之核醣核酸與CMV-NT9 相對應之個別核酸相較,其分子量比CMV-NT9略大,顯示二者可能屬於不同之strain。罹病葉片組織 中,病毒顆粒聚集於細胞質或液胞內呈不規則或結晶狀積聚或則形成內含體。本病毒以蚜蟲進行傳 播試驗結果,桃蚜(Myzus persicae)不具傳播力或傳播能力低。 |
英文摘要 | A cucumovirsu which caused a severe disease on Lisianthus rusellianus in Nan-Tou City during the 1994 growing season was purified and characterized in this study. The infected Lisianthus rusellianus plants showed yellowing, mosaic, and stunting symptoms. Negative stain electron microscopy examination of the crude extract from diseased leaves revealed icosahedral virus particles about 28 nm in diameter. Mechanical infectivity assay using curde saps on 53 plant species from 15 different families showed that the virus was able to infect 27 species of 9 families with 20 species being systemic hosts and 7 local-lesion hosts. The virus was found to infect many species in the family Cucurbitaceae, but it did not infect beans (Phaseolus vulgaris) nor cowpeas (vigna unguiculata). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the purified virus revealed a single coat protein with a relative molecular weight of 29 kDa, same as that found in other cucumoviruses. By ELISA, double-diffusion test, western and tissue blot analysis, the virus is serologically indistinguishable from a well characterized cucumber mosaic virus strain, CMV-NT9. Analysis of the nucleic acid contents revealed that the virus particles contain 4 different RNA species which are slightly larger in size compared to those of CMV-NT9, suggesting that the virus may be a new strain of cucumber mosaic virus. By histological studies, the virus particles were found to aggregate to form irregular or crystal accumulations or inclusion bodies in the cytoplasm or vacuoles of the infected tissues. This virus was not transmitted by Aphis persicae, or it was transmitted at a very low efficiency. |
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