查詢結果分析
來源資料
頁籤選單縮合
題 名 | 康乃馨上洋桔梗壞疽病毒之分離與鑑定=Characterization of Lisianthus Necrosis Virus (genus Necrovirus) Isolated from Dianthus Caryophyllus |
---|---|
作 者 | 陳慶忠; 陳煜焜; 柯文華; 徐惠迪; | 書刊名 | 植物病理學會刊 |
卷 期 | 11:3 2002.09[民91.09] |
頁 次 | 頁137-146 |
分類號 | 435.46 |
關鍵詞 | 康乃馨; 洋桔梗壞疽病毒; 分離; 鑑定; Dianthus caryophyllus L.; Lisianthus necrosis virus isolation; Identification; |
語 文 | 中文(Chinese) |
中文摘要 | 於彰化縣田尾鄉及永靖鄉栽種進口之康乃馨(Dianthus caryophyllus L.)雜交品系“小可愛(Kooij Echo kgr, Holland)”發現一種類似由病毒引起葉片產生淡黃色斑點病徵之植珠。病徵通常於生育中、後期之植株的上方葉片出現許多淡黃色斑點,隨病勢進展許多鄰近斑點融合成斑塊,呈黃色之組織逐漸轉化為壞疸,終致全葉枯萎。罹病葉片粗汁液及超薄片經以電子微鏡觀察到一種直徑約32-33nm之球形病毒,於純化之試料中亦可觀察到類似之病毒顆粒。雙向免疫擴散反應及酵素聯結免疫分析均顯示上述康乃馨所分離之病毒(以下稱康乃馨分離株)的病組織粗汁液或純化病毒與本研究室過去在洋桔梗上所分離之洋桔梗壞疸病毒(Lisianthus necrosis virus, LNV)兔子抗血清產生專一性反應。酵素連結免疫反應分析亦顯示本試驗以康乃馨分離株純化試料製備之老鼠單元抗體與病組織粗汁液抗原產生專一性反應。電泳分析(SDS-polyacrylamide)康乃馨分離株得到一分子量約38kDa的鞘蛋白與LNV之鞘蛋白分子量相似。根據以上證據,本試驗由康乃馨所分離之病毒應屬於LNV之一系統。康乃馨分離株可以系統性感染多種寄生植物,這些寄主並非LNV日本分離株及LNV臺灣洋桔梗分離株的寄主植物或僅是產生局部病斑的寄生植物,顯示康乃馨病毒為LNV之一致病性較強之病毒系統(strain),LNV能感染康乃馨為首次記錄。 |
英文摘要 | A virus was isolated from mini-carnation [Dianthus hybrid Kooij (Echo kgr)] bearing severe viral symptoms. Disease symptoms began on upper leaves with numerous yellow spots, which enlarged and fused into large chlorotic patches expanding to entire leaves. The symptomatic leaves usually became necrotic eventually. Electron microscopy of crude extracts and ultrathin sections of diseased tissues revealed the presence of isometric particles about 32-33 nm in diameter. Similar particles were also purified from diseased tissues of Chenopodium quinoa. Purified virus and extracts of diseased leaves of carnation isolate from C. quinoa reacted positively to Lisianthus necrosis virus (LNV) antiserum in immunodiffusion and indirect ELISA tests. Mouse monoclonal antibodies prepared to the purified virus of carnation isolate reacted positively in both indirect and triple-antibody sandwich ELISA with the extracts of infected leaves of carnation isolate from C. quinoa. The viral capsid protein was approximately 38 kDa based on sodium dodecy1 sulfate-polyacrylamide gel electrophoresis, similar to the size of the LNV coat protein. The virus isolated from carnation is very different biologically from either LNV isolates previously reported in Japan or Taiwan. The carnation isolate experimentally induces systemic infection in many hosts which are either non-hosts or local lesion hosts for LNV. |
本系統中英文摘要資訊取自各篇刊載內容。