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題 名 | 豬皮中低終端冴膠原蛋白之製備=Preparation of Telopeptide-poor Collagen from Pig Skin |
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作 者 | 吳安邦; 鄭慧文; 陳淑茹; 林伶紅; 呂炫堃; 林慶文; 陳朝洋; | 書刊名 | 中華農學會報 |
卷 期 | 187 1999.09[民88.09] |
頁 次 | 頁93-100 |
分類號 | 410.025 |
關鍵詞 | 豬皮; 低終端冴膠原蛋白; 產率; Pig skin; Telopeptide-poor collagen; Yield; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究的目的乃利用本省豐富、價廉的豬皮做為製備低終端�騣早麭J的的來源。當製備條件控制於pH2.5、溫度30 ℃並且分別加入三種不同的1 N酸溶液,所得到的結果為:浸泡49小時後已接近最大的膨脹率,分別為123.9(鹽酸),140.0(檸檬酸),144.6%(乳酸)。在酸溶液中加入胃蛋白�t經消化處理已膨脹的豬皮,所得低終端�騣早麭J白產率分別為40.75(鹽酸),41.06(檸檬酸),41.40%(乳酸)。經純化後的低終端�騣早麭J白,其紅外線光譜顯示保留了三次元構造且並未變性,可順利製備成薄膜,亦可用於開拓生醫材料的新用途。 |
英文摘要 | The objective of this study was to prepare telopeptide-poor collagen by taking the advantages of cheap and abundant pig skin produced in Taiwan. The conditions for the preparation were adjusted to 30 ℃ and pH 2.5. After separately adding three different acidic solutions (1 N) and soaking for 49 hours, maximum swelling ratios were almost attended: the results were 123.9 (hydrogen chloride), 140.0(citric acid), 144.6%(lactic acid), respectively. The swelled pig skin was further treated with acidic solution plus pepsin for digestion. The yields for telopeptide-poor collagen were: telopeptide-poor collagen exhibits the retention of three dimensional structure without denaturation. The collagen can be prepared successfully in the form of which is suitable for the special uses as bio-medical materials. |
本系統中英文摘要資訊取自各篇刊載內容。