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題名 | The Analysis of Polycyclic Aromatic Hydrocarbons Derived from Cooking Fumes by HPLC with UV Detection and Wavelength Programming=以高效率液相層析法分析烹飪油煙中多環芳香烴類化合物之最佳分析條件研究 |
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作者 | 吳佩芬; 姜泰安; Wu, Pei-fen; Chiang, Tai-an; |
期刊 | 大仁學報 |
出版日期 | 19990500 |
卷期 | 17 1999.05[民88.05] |
頁次 | 頁185-198 |
分類號 | 343.38 |
語文 | eng |
關鍵詞 | 多環芳香烴; 烹飪油煙; 高效率液相層析法; Polycyclic aromatic hydrocarbons; Cooking fumes; High performance liquid chromatography; |
中文摘要 | 本研究以高效率液相層析法分析烹飪油煙中多環芳香烴類化合物含量,為了選擇最佳之分析條件,本研究將分析條件分為有機溶劑萃取回收率與UV偵測器潻長選擇來分析烹飪油煙樣本在何種條件下之靈敏度(sensitivity)與鑑別度(selectivity)有最佳的表現。其中在有機溶劑萃取回收率分析中選取四種有機溶劑(acetone、isooctane、acetonitrile與dichloromethane)來分析九種多環芳香烴類化合物回收率數值,結果顯示acetone、isooctane與acetonitrile之萃取回收率均達75%以上,而dichloromethane之萃取回收率則低於70%以下,在四種有機溶劑中以acetone之萃取回收率最高平均達81.2%。在UV偵測器波長選擇分析方面,本研究將偵測波長分成單一波長(λ=254 nm)與變動波長兩種,結果顥示以單竹波長(λ=254nm)來分析多環芳香烴類化合物含量之偵測極限值為0.2∼2ng,而若以變動波長來分析多環芳香烴類化合物含量之偵測極限值則可降低為為0.1∼0.5ng,顯示以變動波長來分析多環芳香烴類化合物含量之靈敏度較佳。而本研究採取之C�睆猻W亦顯示出相當好之鑑別度,完全將九種多環芳香烴類化合物分別開來,本研究為了避免多環芳香烴類化合物之滯留時間(retention time)有偏移的現象,並同時計算以benzo(a)pyrence為標準之滯留時間指標(retention time index, PTI),結果顯示其再現性相當高(98.7%),將可增加多環芳香烴類化合物之鑑別度。再將上述分析所得之高效率液相層析法之最佳分析條件實際應用在分析烹飪油煙中多環芳香烴類化合物含量上,結果顯示烹飪油煙中含有anthracene、fluoranthene、pyrene、benzo(a)anthracene、chrysene,benzo(b)fluoranthene、benzo(k)fluoranthene、benzo(a)pyrene與dibenz(a,h) anthracene 等九種多環芳香烴類化合物,其濃度範圍為0.5 ∼22.1 ug/m�纂A然而若使用傳統單一波長(λ=254 nm)來分多環芳香烴類化合物含量則有anthracene,fluoranthene與chrysene未能 偵測出來,且濃度均較前述方法為低。本研究所分析出來之高效率液相層析法之最佳分析條件應可有效提昱多環芳香烴類化合物或含量分析之靈敏度與鑑別度,並可應用於烹飪油煙之 環境暴露劑量監測研究。 |
英文摘要 | The present study analyzed nine polycyclic aromatic hydrocarbons (PAHs) by high performance liquid chromatography (HPLC) with ultraviolet detection in cooking fumes. In order to optimized the analysis procedures, HPLC analysis with constant wavelength (λ=254nm) or programming wavelengths through a 5- um polymeric C�� column. the air- sampling filters wrer extracted with Soxhlet apparatus using several organic solvents to compare their recoveries. The bibliography data suggested that this kind of stationary phase yield to the PAHs best selectivity and totally separeated on a LC-PAH Supelcosil column with an acetonitrile-water elution gradient. The retention time index (RTI) values for standards, benzo(a)pyrene, were used to identified PAHs and found to be highly reproducible (98.7%)and as a more reliable means of identification. All the chosen PAHs were recovered at more than 75% when using acetone, isooctane, and acetonitrile as extraction solvents. These recoveries were however less than 70% when dichloromethane were the extraction solvents. Acetone showed the best efficacy of extraction among these four organic solvents with the average recoveries of 81.2%. The detection limit at λ=254 nm varied along from 0.2 to 2 ng. However, the detection limits of PAHs using programming wavelengths were ranged from 0.1 to 0.5ng. The programming wavelengths programming wavelengths weere ranged from 0.1 to 0.5 ng. The programming wavelengths during the HPLC analysis improved both sensitivity and selectivity. The PAH contents in cooking fumes were performed by the optimum method described above; extracted by acetone and analyzed by HPLC at programming wavelengths. All extracts of cooking fumes contained anthracene, fluoranthene, pyrene, bezo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, and dibenz(a,h) anthracene at the concetrations ranging from 0.5 to 22.1 g/m��.However, anthracene, fluoranthene, and chrysene could not be detected when using a single wavelength(λ=254nm). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。