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題名 | 利用毛細管電泳定量中藥製劑成分(3)=Determination of the Contents in Chinese Herbal Preparations by Capillary Electrophesis(3) |
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作 者 | 許順吉; | 書刊名 | 中醫藥年報 |
卷期 | 17:2 1999.05[民88.05] |
頁次 | 頁555-621 |
分類號 | 414.33 |
關鍵詞 | 中藥製劑; 芍藥湯; CE; HPLC; Chinese herbal preparation; Guanosine; Dehydro evodiamine; Ephedrine; Sinomenine; Shao-yao-tang; |
語文 | 中文(Chinese) |
中文摘要 | 高效液相層析(HPLC)及毛細管電泳(CE)是目前最常用來定量中藥指標成份的分析 方法。本研究以這兩種儀器,分析含半夏、吳茱萸、麻黃及防己的方劑,及芍藥湯製劑,並 比較這兩種方法的優劣。 十一件含半夏方劑的 guanosine 含量, 以磷酸鹽溶液沖提, 用 HPLC 分析,一小時可完成;以 borate 為緩衝溶液用 CE 分析,只需時間十二分鐘。 應用 於市售製劑,HPLC 可分析七製劑,CE 可分析九製劑,其餘解析度不太理想。兩件含吳茱萸 方劑的 dehydroevodiamine 含量,以磷酸鹽沖提, 用 HPLC 分析,分析時間四十五分鐘; 以磷酸二氫鈉為緩衝溶液,用 CE 分析,需時六分鐘。兩方法用於市售製劑,效果良好。十 一件含麻黃方劑的 ephedrine 含量,以含 SDS 的磷酸溶液沖提,用 HPLC 分析,耗時五十 分鐘;以 vaimne 的氨水溶液遷送,用 CE 分析,需時四分鐘。後者可順利用於十一市售製 劑,前者有兩製劑分析效果不太理想。 以含 SDS 的酒石酸水溶液沖提,用 HPLC 分析小續 命湯 (含防己 ) 中的 sinomenine 含量,需時六十五分鐘; 以含 malic acid 磷酸二氫鈉 溶液遷送,用 CE 分析該方劑,需時七分鐘。芍藥湯由芍藥、黃苓、大黃、黃連、肉桂、木 香、當歸及檳榔等藥材剪煮而得,其組成成分,可順利用 HPLC 及 CE 分析,前者以酸鹽為 沖提,後者以硼酸鹽及 SC 為緩衝液。 |
英文摘要 | High-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are currently the most commonly used analysis methods for assaying the marker substances in Chinese herb drugs. In this study the two methods were used to analyze the preparations containing Pinelliae Tuber, Evodiae Fructus, Ephedrae Herba and Stephaniae Radix. Fourteen samples of Chinese herb formulas containing Pinelliae Tuber were assayed for their guanosine contents, using phosphate eluent in the HPLC analysis completed within one hour, and using borate buffer in the CE method taking only 12 minutes. Among the preparations, HPLC can analyze eight, and CE, nine. The other preparations came out with poor resolution. Two evodia-containing herbal preparations were tested for their dehydroevodiamine contents, using phosphate eluent in the HPLC method. The analysis took 45 minutes. While sodium dihydrophosphate buffer solution was used in the CE method to take only six minutes. Both methods gave satisfactory results. Eleven ephedra-containing herbal preparations were tested for the ephedrine content, using SDS-phosphate solution as the eluent in HPLC method, which took 50 minutes. Whereas valine-ammonia solution was used in the CE method which took only 4 minutes. The latter coul dsuccessfully analyze eleven commercial herbal preparations, while the former could analyze only nine. With the SDS-tartaric acid water solution as the eluent in the HPLC method for analyzing the herbal formula Hsiao-hsu-ming-tang (Ma-huang and Peony Combination), it took 65 minutes. Alternatively, CE, using malic acid and sodium dihydrophosphate solution for analyzing the same formula, took only seven minutes. Shao-yao-tang (Peony Combination) is composed of Paeoniae Radix, Scutellariae Radix, Rhubarb Rhizoma, Cinnamomi Cortex, Saussureae Radix, Angelicae Radix and Arecae Fructus, and can be easily analyzed with HPLC and CE methods. The former uses phosphate as eluent and the latter uses boric acid and SC as a buffer. |
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