查詢結果分析
來源資料
相關文獻
- Heparin Immobilization onto Polyurethane Surfaces and Their Characterization
- The Role of Heparin in Treatment of Purpura Fulminans: Experience in Four Cases
- Corneal Opacity and Congenital Glaucoma Associated with Massive Heparan Sulfaturia: Report of One Case
- 肝素及Ticlopidine於犬血液透析之抗凝研究
- 肝素及低分子量肝素對急性肺栓塞的治療
- The Influence of Intra-Graft Heparin Injection on the Survival of Composite Grafts
- A Comparative Study of Urokinase and Heparin in the Treatment of Deep Vein Thrombosis
- Evaluation of a Heterologous Pericardium Immobilized with Heparin as a Vascular Patch in a Canine Model
- 犬血液透析時血液流速與肝素劑量之相關研究(1)
- 肝素的化學構造及其性質
頁籤選單縮合
題名 | Heparin Immobilization onto Polyurethane Surfaces and Their Characterization=肝素接枝在聚胺脂表面及其鑑定分析研究 |
---|---|
作者 | 王水深; 林永生; 周迺寬; 朱樹勳; | 書刊名 | 中華民國外科醫學會雜誌 |
卷期 | 31:6 民87.11-12 |
頁次 | 頁351-361 |
分類號 | 416.26 |
關鍵詞 | 肝素; 聚胺脂; Polyurethane; Heparin; Photochemical reaction; Polyethyl-eneimine; Blood compatibility; |
語文 | 英文(English) |
中文摘要 | 聚胺脂(polyurethane,PU)已被廣泛應用在人工心臟及人工血管等生物醫學材料領域而有良好的血液相容性,然而在活體評估中,纖維蛋白亦常於 PU 表面上形成。為了左心室輔助器(LVAD)的研製及PU人工血管的臨床運用,其血液相容性,有需要進一步提升。在本文中,我們成功的將肝素(heparin)利用共價鍵接枝到PU表面上,以增加其生体相容性。 我們利用光化學反應和傳統化學鍵結法將肝素接枝到PU表面,以減少肝素逐漸從PU表面上逸出,並可保留肝素接枝到PU表面後之活性。光化學反應接枝步驟如下:首先將肝素與苯基疊氮化合物(phenly azide)反應成肝素-疊氮化合物(heparinazide即photohep);而polyethylene glycol(PEG)則另先接枝到PU表面形成PU-PEG。接著再運用光化學反應將photohep接枝到PU-PEG表面上,最後形成PU-PEG-photohep表面結構。 肝素接枝之PU表面經化學分析電子儀(ESCA)硫及碳信號分析,證實本研究已成功將肝素接枝到PU表面並製備了三種不同接枝之PU表面,即PU-六次甲基二異氰酸鹽(hexamethylene diisocyanate即HMDI)-聚乙亞胺(PEI)-PEG-photohep,PU-HMDI-PEG-photohep及PU-HMDI-PEG-HMDI-heparin。由ESCA和肝素定量分析,我們發現光化學反應法較一般傳統化學鍵結法有較高之肝素接枝在PU表面。而PEI可有效的促進光化學反應將肝素接枝在PU表面。 |
英文摘要 | Polyurethane (PU) has been widely used as biomaterial in artificial heart and vascular graft applications and reveals effective blood compatibility. However, PU still does not show excellent blood compatibility because fibrin formation is evident after in vivo evaluation. In this paper, heparin was covalently immobilized onto the surface of PU to increase the biocompatibility. Both photochemical method and traditional chemical bonding were used to couple heparin onto PU surfaces to decrease heparin release from the surfaces and finally to maintain the activity of immobilized heparin molecules. The basic procedures for photochemical immobilization were as follows. First, heparin reacted with phenyl azide to become heparin-azide; polyethylene glycol (PEG) was coupled onto the PU surface to become PU-PEG respectively. Second, by photochemical reaction, heparin-azide (photohep) was immobilized onto the PU-PEG surfaces and became a PU-PEG-photohep surface structure. Our results showed that heparin was successfully coupled onto PU-hexamethylene diisocyanate (HMDI)-polyethyleneimine (PEI)-PEG-photohep and PU-HMDI-PEG-photohep via photochemical method, and PU-HMDI-PEG-HMDI-heparin surfaces via traditional chemical bonding. From the results of the sulfur and carbon signals by Electron Spectroscopy for Chemical Analysis (ESCA) and heparin quantitative analysis, we found that photochemcal method immobilized more heparin on PU surfaces, and PEI could greatly enhance the photochemically immobilized heparin density. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。