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- 魚油在含膽固醇之飼料中提高倉鼠低密度脂蛋白膽固醇濃度卻不改變肝臟低密度脂蛋白受體mRNA之表現
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頁籤選單縮合
題名 | 魚油在含膽固醇之飼料中提高倉鼠低密度脂蛋白膽固醇濃度卻不改變肝臟低密度脂蛋白受體mRNA之表現=Fish Oil in a Cholesterol Containing Diet Elevates LDL-Cholesterol Without Changing LDL-Receptor mRNA in Hamsters |
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作者 | 彭郁玲; 呂紹俊; 黃伯超; Peng, Yu-ling; Lu, Shao-chun; Huang, Po-chao; |
期刊 | 中華民國營養學會雜誌 |
出版日期 | 19980800 |
卷期 | 23:3 1998.08[民87.08] |
頁次 | 頁309-322 |
分類號 | 437.111 |
語文 | chi |
關鍵詞 | 魚油; 膽固醇; 低密度脂蛋白; 低密度脂蛋白受體mRNA; 倉鼠; Fish oil; Cholesterol; LDL; LDL-receptor mRNA; Hamsters; |
中文摘要 | 這個實驗是以不含或含0.1%膽固醇飼料探討添加0%、1.5%、3%及4.5%魚油對倉鼠血漿脂肪、肝臟脂肪及肝臟低密度脂蛋白受體(LDL-receptor)mRNA 表現量的影響。實驗是將40隻重約50g之離乳雄性黃金倉鼠(Golden Syrian hamster)平均分為八組,分別餵養上述之實驗飼料為期四週。 攝食不添加膽固醇飼料的倉鼠, 血漿總三酸甘油酯(Total-TG)、超低密度脂蛋白三酸甘油酯(VLDL-TG)、總膽固醇(Total-C)及低密度脂蛋白膽固醇(LDL-C)隨魚油量的增加有顯著的下降(P<0.05),但魚油對肝臟三酸甘油酯及膽固醇則影響不大。而攝食添加0.1%膽固醇飼料的倉鼠,血漿Total-TG及VLDL-TG則只有4.5%魚油組才明顯的低於0%魚油組(P<.0.05)。但Total-C及LDL-C則受魚油影響而明顯升高,其中LDL-C更由42.2 ± 2.2升高至108.5 ± 18.4 mg/dL。飼料添加0.1%膽固醇使肝臟膽固醇增加1∼3倍,但魚油卻使肝臟膽固醇增加幅度減低;而魚油也使肝臟三酸甘油酯顯著的降低。飼料中添加0.1%膽固醇明顯使倉鼠肝臟LDL-receptor mRNA減少約一半,但4.5%魚油卻對LDL-receptor mRNA表現量沒有影響。這些結果顯示,魚油對倉鼠脂質的代謝會受到食物中膽固醇的影響而有改變,飼料含0.1% 膽固醇時魚油飼料引起的LDL-C升高並不是因為LDL-receptor mRNA表現減少所導致,而真正的原因仍有待進一步的探討。 |
英文摘要 | This study was designed to investigate the effect of 0%, 1.5%, 3% or 4.5% fish oil diet on plasma lipids, liver lipids and liver LDL-receptor mRNA expression with or without supplementation of 0.1% dietary cholesterol. Forty Golden Syrian hamsters weighing about 50g were divided into 8 groups and were fed with one of the experimental diets for 4 weeks. In hamsters consuming diets without cholesterol supplementation, plasma total-triglyceride (TG), VLDL-TG, total-cholesterol (C) and LDL-C decreased gradually as fish oil increased in the diet; but liver triglyceride and cholesterol were not significantly changed by fish oil. While in 0.1% cholesterol dietary groups, only 4.5% fish oil group had significantly lower plasma total-TG and VLDL-TG than the 0% fish oil group; however, 1.5%, 3% and 4.5% fish oil groups all had higher total-C and LDL-C than the 0% fish oil group. The elevation of LDL-C was especially remarkable, from 42.2 ± 2.2 of the 0% fish oil diet to 108.5 ± 18.4 mg/dL of the 4.5% fish oil diet. Addition of 0.1% cholesterol in the diet resulted in significant increase of liver cholesterol and decrease of LDL-receptor mRNA. The accumulation of liver cholesterol was lowered by fish oil, but LDL-receptor mRNA in 4.5% fish oil group was similar to that in 0% fish oil group. These data suggest that the effects of fish oil on lipid metabolism can be changed by dietary cholesterol. Also the elevation of LDL-C by fish oil in cholesterol containing diet is not due to low expression of LDL-receptor mRNA. The mechanism behind these changes is still not clear and requires further investigation. |
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