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題名 | 原型及固定化乳糖酶在乳糖水解過程中寡糖形成之比較=Oligosaccharide Formation in the Process of Lactose Hydrolysis by Native and Immobilized Lactases |
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作者 | 陳建元; 曾浩洋; Chen, Jan-yuan; Tsen, Hau-yang; |
期刊 | 技術學刊 |
出版日期 | 19981200 |
卷期 | 13:4 1998.12[民87.12] |
頁次 | 頁647-652 |
分類號 | 346.215 |
語文 | chi |
關鍵詞 | 乳糖酶; 固定化; 寡醣形成; Lactase; Immobilization; Oligosaccharide formation; |
中文摘要 | 不同來源乳糖�t在25%乳糖溶液中之寡醣最高形成量之大小,依序為Bacillus circulans 乳糖�t> Kluyveromyces fragilis 及 Saccharomyces lactis 乳糖�t> Aspergillus oryzae 乳糖�t。 以不同方法固定化 B. circulans 乳糖�t,其寡醣最高形成 量之大小依序為瓊脂醣、矽膠嵌入化乳糖�t>原型乳糖�t>纖維三醋酸酯嵌入化乳糖�t。A. oryzae、K. fragilis 及 B. circulans 等乳糖�t形成二醣及三醣之模式相似。 |
英文摘要 | During the enzymatic hydrolysis of lactose, oligosaccharides could be simultaneously synthesized. The order of enzyme sources for maximum oligosaccharide formation was Bacillus circulans > Kluyveromyces fragilis, and Saccharomyces lactis > Aspergillus oryzae. Methods of enzyme immobilization could affect the quantities of oligosaccharide formed. In the case of B. circulans lactase, the order of oligosaccaride formation based on the enzyme forms was agarose and silica entrapped > native > fiber entrapped. The mechanism for the formation of disaccharides and trisaccharides for lactases from A. oryzae, K. fragilis and B. circulans lactases were similar. |
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