查詢結果分析
來源資料
相關文獻
- 纖維嵌入固定化Bacillus Circulans乳糖酶之特性及動力學
- A Study of the Tree and Wood Characteristics of Young Pedigreed Jack Pine
- 乾濕梅雨季東亞環流特性之研究
- 臺北市廢棄煤礦礦區棄土場植生特性之研究
- Penicillium simplicissimum W46胞外植酸酶之生產、純化及特性分析
- 臺灣地區大學圖書館館員工作滿意度影響因素探討
- 營養生長期的短期土壤缺水對百合生育之影響
- 氣渦輪機使用液態空氣之進口空氣冷卻系統
- 以聚苯乙烯和含羥基之苯乙烯共聚物為共膜材料製備乙基纖維素微膠囊及其制放行為研究
- 臺電底灰性質之探討
頁籤選單縮合
題名 | 纖維嵌入固定化Bacillus Circulans乳糖酶之特性及動力學=Characteristics and Kinetics of Fiber Entrapped Bacillus Circulans Lactase |
---|---|
作者 | 陳建元; 曾浩洋; | 書刊名 | 技術學刊 |
卷期 | 12:3 1997.09[民86.09] |
頁次 | 頁517-521 |
分類號 | 346.215 |
關鍵詞 | 乳糖酶纖維嵌入固定化; 特性; 動力學參數; Lactase fiber entrapped; Properties; Kinetic parameters; |
語文 | 中文(Chinese) |
中文摘要 | 將且B. circulans來源的乳糖醜以嵌入法固定於纖維三醋酸醋(CTA)中。以 50units/gCTA之酵素用量在600rpm之乳化速度下所製得之纖維嵌入化B. circulans乳糖□ ,其活性效率最高。以5%乳糖溶液為基質,在200rpm振盪速率下所測得之活性效率可達 2%左右。B. circulans乳糖□經纖維嵌入固定化後,其酵素特性包括最適作用溫度、pH 及熱穩定性等,均與原酵素相近。由於基質擴散限制(diffusion limitation)之原因,纖 維固定化酵素之Km值比原酵素大10倍,各為115mM及l1.2mM。在50mM半乳糖存在下,纖維 嵌入化B. circulans乳糖□及其原酵素之Ki值則分別為285mM及163mM,而Lineweaver- Burk圖形顯示半乳糖為B. circulans乳糖□之競爭性抑制劑。 |
英文摘要 | Bacillus circulans lactase was entrapped into cellulose triacetate ( CTA) fibers. The optimal conditions for enzyme entrapment were as follows. The ratio of enzyme to CTA was 50 units to 1 g and the emulsifying speed was 600 rpm. When a 5% lactose solution was used as the substrate, at a 200 rpm shaking speed, the activity efficiency could reach 32%. Enzyme properties, such as the optimal temperature, optimal pH and thermal stability of cellulose triacetate entrapped B. circulans lactose, were similar to those of the enzyme's native form. Due to the diffusion limitation for the substrate, the Km of the fiber entrapped enzyme was about 1 0 times that of its soluble form, i.e., 115 mM vs 11.2 mM, when lactose was used as the substrate. In the presence of 50 mM galactose, the Ki of the native and immobilized B. circulans lactase were 163 mM and 285 mM, respectively. In addition, the Lineweaver-Burk plot shows that galactose is the competitive inhibitor for lactase. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。