頁籤選單縮合
題 名 | 七寶美髯丹的抗氧化作用之研究=The Study on the Anti-Oxidative Effect of Chi-Bau-Mei-Ran-Dan |
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作 者 | 陳明豐; | 書刊名 | 中醫藥年報 |
卷 期 | 16:2 1998.05[民87.05] |
頁 次 | 頁189-207 |
分類號 | 414.33 |
關鍵詞 | 七寶美髯丹; 抗氧化作用; 超氧陰離子; 過氧化氫; 過氧化脂質; Chi-bau-mei-ran-dan; Anti-oxidative effect; Superoxide anion; Hydrogen peroxide; Lipid peroxide; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究目的乃探討七寶美髯丹之抗氧化作用。我們以Cytochrome C法 及I.N.T.法測定七寶美髯丹在試驗管內清除xanthine/xanthine oxidase產 生之superodixe anion(O��)的作用。另一方面,我們也以lucigenin 及 luminol之微量化學發光法測定七寶美髯丹清除白血球產生之O�砟咠�烙�祖漣@ 用。更進一步地則探討七寶美髯丹在實驗管內抑制FeCl�粉今o大白鼠肝細胞 脂質過氧化的作用。 結果顯示,不論 以cytochrome C法或I.N.T.法測定,七寶美髯丹都明 顯地清除xanthine/xanthine oxidase 產生的O�砥A且呈劑量關係。另一方面, 七寶美髯丹也有意義地清除非刺激狀態及刺激狀態下白血球產生的O�砟� H�烙�砥C除此之外,七寶美髯丹也顯著地抑制實驗管內FeCl�粉今o大白鼠肝細 胞過氧化脂質的產生。以上結果顯示七寶美髯丹具有良好的抗氧化作用, 其抗氧化作用的來源,值得進一步探討。 |
英文摘要 | The aim of this study is to investigate the anti-oxidative effect of Chi-Bau-Mei-Ran-Dan (CBMRD), a Chinese herb drug prescription. We evaluated the scavenging effect of CBMRD on superoxide anion (O��) produced by xanthine/xanthine oxidase with cytochrome C method and I.N.T. method. On the other hand, we also measured the scavenging effect of CBMRD on the O�畝nd hydrogen peroxide (H�烙��)produced by leukocytes with chemiluminescence analyzer and amplisensors including lucigenin and luminol. Furthermore, we investigated the inhibitory activity of CBMRD on the production of rat liver homogenate induced by FeCl��. The results revealed that, in both cytochrome C method and I.N.T. method, CBMBD significantly reduced O�� produced by xanthine/xanthine oxidase with a dose-dependent relation. On the other hand, CBMBD markedly scavenged the O�皰roduced by leukocytes under both unstimulated and stimulated state. Moreover, CBMBD also significantly inhibited the production of lipid peroxide from rat liver homogenate induced by FeCl�疳n vitro. The above results suggest that CBMRD has good anti-oxidative effects. It is worthful for us to further investigate the origin of anti-oxidative effects of CBMRD in future. |
本系統中英文摘要資訊取自各篇刊載內容。