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| 題名 | Decoloration of Azo Dye by Immobilized Wood Rotting Fung=固定化之木材腐朽真菌對偶氮染料脫色能力的探討 |
|---|---|
| 作者 | 趙維良; 王美惠; Chao, Wei-liang; Wang, Mei-huey; |
| 期刊 | 中國農業化學會誌 |
| 出版日期 | 19980200 |
| 卷期 | 36:1 1998.02[民87.02] |
| 頁次 | 頁21-30 |
| 分類號 | 369.4 |
| 語文 | eng |
| 關鍵詞 | 偶氮染料; 木材腐朽真菌; 固定化; 褪色; Azo dye; Wood rotting fungi; Immobilization; Decoloration; |
| 中文摘要 | 本文所用的主要測試菌株SM7是腐朽的木材分離而得，此株菌不論培養在高氮、低 氮或馬鈴薯葡萄糖培養基中均能有效的使偶氮染料（ orange G ）脫色。 如將菌絲利用 sodium alginate 予以固定後加入 orange G 水溶液中，每隔 24 小時加入新的染液，到第 五天時這些固定化的菌絲已喪失大部份的脫色能力； 反之，在含有 orange G 的 dimethyl succinate 緩衝液中，我們並未觀察到這些固定化菌絲喪失其脫色的能力。當褪色能力下降 時，我們同時觀察到 pH 值的上升。如以玉米穗軸來培養菌株 SM7，同樣的我們也看到良好 的脫色結果，在 16 小時內可將 96 ％的加入染劑褪色。 在菌株 SM7 的培養上清液中，所 測得的木質素分解酵素以 laccase 為主，在漂洗過的菌絲上則只測到 laccase 的存在。當 用漂洗過的菌絲來進行脫色實驗時，此僅用上清液具有較好的脫色能力，但比兩者同時存在 時要差。可能是因為上清液中缺乏由菌絲所產生的過氧化氫。此外，菌絲上可能還有其他的 脫色酵素，不在我們的測定項目中。 菌株 SM7 經 International Mycological Institute Biosystematic Service 鑑定屬於層菌綱，但因無法誘使其產生有性世代，故無法得知其確 實分類地位。 |
| 英文摘要 | The test fungus, strain SM7, was isolated from a piece of rotted wood. This organism had excellent ability to decolorize azo dye (e. g., orange G) regardless of whether it was pre-cultured in nitrogen-limited, nitrogen-rich or potato dextrose broth. Fungal hyphae was immobilized by using sodium alginate. When the immobilized fungus, either as alginate beads or capsules, was added to water containing orange G, significent reductions in their decoloration abilities were observed at the end of the experiment. On the other hand, when dimethyl succinate buffered orange G solution was used, the test fungus maintained its decoloration ability for a longer peroid of time. The decrease of its decoloration ability coincided with an increase of the pH value in the system. When strain SM7 was pre-cultured on corn cob pieces, excellent decoloration results were achieved even after repeated addition of the dye solution, and more than 96% of the dye was decolorized in 16 hours. The major ligninolytic enzyme in strain SM7 culture supernatant was laccase, and very little or no lignin peroxidase and manganese dependent peroxidase was detected. On washed hyphae, laccase was the only lignin-degrading enzyme observed. However, when washed hyphae was used in the decoloration study, it decolorized orange G faster than did the culture supernatant alone but slower than did the complete culture (i. e., hyphae plus supernatant). We believe this was caused by a lack of hydrogen peroxide, which is produced by the enzyme located in the periplasmic space. However, we can not completely rule out the possibility that there were other enzymes on the hyphae which could decolorize orange G. Strain SM7 was identified as one of the Hymenomycetes by the International Mycological Institute Biosystematic Service. |
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