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| 題 名 | Structure, Regulation and Evolution of Vertebrate Lactate Dehydrogenase Genes=脊椎動物乳酸去氫酶基因之構造、調控及進化 |
|---|---|
| 作 者 | 李水龍; | 書刊名 | 動物研究學刊 |
| 卷 期 | 37:1 1998.01[民87.01] |
| 頁 次 | 頁1-6 |
| 分類號 | 364.64 |
| 關鍵詞 | 乳酸去氫酶; 異構酶; 遺傳基因; 分子演化; Genes; Isozymes; Protein sequences; Phylogenetic tree; |
| 語 文 | 英文(English) |
| 中文摘要 | 在脊椎動物中L-乳酸去氫 的異構 A(主要表現於肌肉)及B(主要表現於心臟)各 是研究乳酸還原及乳糖氧化最適當的蛋白。在哺乳類及哥倫比亞鳥中發現了第三種乳酸去 氫 ,其表現在睪丸中。在高等的真骨魚類中這種第三類乳酸去氫 同時在肝及眼中發現, 但在低等的真骨魚類中,此蛋白則廣泛的分布在體內。這三種乳酸去氫 在演化上之間的關 係並沒有完全的解決。 我及我的研究同仁已選殖了多種生物的乳酸去氫 A,B,C三種異構 ,包括人類, 鼠,大白鼠,豬,鴿,鱷魚,蜥蜴,龜,非洲爪蟾及線蟲。並決定了人類乳酸去氫 A基因 的內-外子構造及定序了鼠的完整的乳酸去氫 A基因之12851核酸長度的序列。哺乳類動 物及鴨子的乳酸去氫 A,B,C基因的蛋白表現區域被6個內子分割在相似的位置。脊椎 動物的乳酸去氫 A,B,C表現為器官專一性且在細胞分化上受到調控。而哺乳類的乳酸 去氫 A的表現已證明受到Camp及estrogen的激化。 在與李文雄博士的合作中,我們分析了脊椎動物中各種乳酸去氫 異構 之間的演化關 係。相對於傳統上相信龜的分支早於蜥蜴/鱷魚及鳥的分支,我們發現龜的分支同於鱷魚或 鱷魚/鳥的分化支,而蜥蜴的分支則早於鱷魚/龜及鳥的分支。鴿子睪丸的乳酸脫氫 C則明 顯為複製於乳酸脫氫B,故其不同於哺乳類的乳酸脫氫 C,因為在哺乳類中其衍生於乳酸 去氫 A。 |
| 英文摘要 | Steven Shoel-Lung Li (1998)Structure, regulation and evolution of vertebrate lactate dehydrogenase genes. Zoological Studies 37(1): 1-6. In vertebrates, L-lactate dehydrogenase (LDH) isozymes A (muscle) and B (heart) are best suited for pyruvate reduction and lactate oxidation, respectively. In mammals and columbid birds, a 3rd LDH-C isozyme is expressed in testis. In advanced teleost fish a 3rd LDH isozyme is found only in the eye, but in more primitive teleosts, if it is present, it has a generalized distribution. The evolutionary relationships among these 3rd forms of LDH isozymes have not been completely resolved. We have cloned the cDNAs encoding LDH-A, LDH-B, and/or LDH-C isozymes from the human, mouse, rat, porcine, pigeon, alligator, lizard, turtle, Xenopus, and a mematode. We have determined the exon-intron organization of human LDH-A, LDH-B, and LDH-C genes, and the complete sequence of 12 851 nucleotides of the mouse LDH-A gene. The protein-coding sequences of the mammalian LDH-A, LDH-B, and LDH-C genes, as well as duck LDH-B gene, are interrupted by 6 introns at homologous positions. The expression of vetebrate LDH-A, LDH-B, and LDH-C genes is tissue specific and developmentally regulated. Expression of the mammalian LDH- A gene was also shown to be induced by camp and estrogen. In collaboration with Dr. Wen-Hsiung Li, we analyzed the evolutionary relationships among vertebrate LDH isoyzmes. Contrary to the common opinion that the turtle lineage branched off before the divergence between the lizard/alligator and bird lineages, the turtle lineage was found clustered with either the alligator lineage or the alligator-bird clade, while the lizard lineage was found to have branched off before the divergence between the alligator/turtle and bird lineages. The pigeon testicular LDH-C gene was evidently duplicated from the LDH-B (heart) gene, so it is not orthologous to the mammalian testicular LDH-C genes, which appear to be derived from the LDH-A (muscle) gene. |
本系統中英文摘要資訊取自各篇刊載內容。