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題名 | 禽、畜飼料中甲磺黴素及氯黴素之同時檢測=Simultaneous Determination of Thiamphenicol and Chloramphincol in Porcine and Poultry Feeds |
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作者姓名(中文) | 郭美月; 劉敏主; 林士鈺; | 書刊名 | 臺灣省家畜衛生試驗所研究報告 |
卷期 | 33 1997[民86.] |
頁次 | 頁117-123 |
分類號 | 437.111 |
關鍵詞 | 甲磺氯黴素; 氯黴素; 高效液相層析法; Thiamphenicol; Chloramphenicol; High performance liquid chromatographic method; |
語文 | 中文(Chinese) |
中文摘要 | 應用高效液相層析法( High performance liquid chromatographic method ) 同時檢測飼料之中甲磺氯黴素( Thiamphenicol; TP )及氯黴素( chloramphenicol;CM )。 大豬及蛋雞飼料檢體分別以乙晴抽取, 再以 SEP PAK Alumina N 卡匣純化後以 TSK-GEL ODS-120 T 為層析管,乙晴 -0.05% 磷酸( 18-82 )為移動相, 以瞬間改變波長 的方式在 230 nm 及 280 nm 偵測下可得良好之分離效果及線性關係之檢量線。大豬飼料中 TP 及 CM 之回收率為 76.9 % 及 73.6 %。 蛋雞飼料中 TP 及 CM 之回收率分別為 76.2% 及 65.8%。 |
英文摘要 | A Hight performance liquid chromatographic method for simultaneous determination of thiamphenicol ( TP ) and chloramphenicol ( CM ) in porcine and poultry feeds has been developed. These feeds were extracted with acetonitrile, then purified with SEP PAK alumina N cartridge. With TSK-GEL ODS-120 T column and acetonitrile: 0.05% phosphoric (18:82) as mobile phase, a good seperation and linear calibration curves of TP and CM could be obtained by being detected at 230 nm switching to 280nm. Recoberies of TP and CM in porcire feeds were 76.9% and 73.6%, respertively. Recoberies of TP and CM in poultry feeds were 76.2% and 65.8%, respertively. |
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