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題名 | 生體外β-胡蘿蔔素的添加對黃麴毒素B1所引發的豬隻周邊血液淋巴細胞和肺泡巨噬細胞致害的保護作用=Protection of β-carotene on Aflatoxin B1-induced Swine Peripheral Blood Lymphocyte and Alveolar Macrophage Toxicity in Vitro |
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作 者 | 吳繼芳; 鄭永祥; | 書刊名 | 中華農學會報 |
卷期 | 180 1997.12[民86.12] |
頁次 | 頁92-104 |
分類號 | 437.65 |
關鍵詞 | 淋巴細胞; 肺泡巨噬細胞; 黃麴毒素B1; β-胡蘿蔔素; 豬; Lymphocytes; Alveolar macrophage; Aflatoxin B1; β-carotene; Swine; |
語文 | 中文(Chinese) |
中文摘要 | 本實驗旨在探討生體外β-胡籮蔔素(BC)的添加對黃麴毒素B1(AFB1)所引發的豬隻 周邊血液淋巴細胞(PBL)和肺泡巨噬細胞致害的保護作用。 實驗一、生體外BC添加對黃麴毒素B1所引發的PBL之DNA, RNA和蛋白質合成作用干擾 之影響。實驗結果顯示AFB1(5 ng/ml)對照組(D組)雖對DNA合成稍有抑制趨勢,但此差異不 具統計意義,而BC(0.2μM/ml)添加可稍微改善AFB1對DNA合成的抑制作用。BC添加可有效 改善AFB1、BC及PBL同時加入培養(A組)和BC先與PBL培養2小時後,再加入AFB1(B組)之 RNA合成抑制作用,且改善至與培養基對照組(F組)之結果相同。BC添加的時間對RNA合成 具有決定性影響,由AFB1與PBL培養2小時後再加BC(C組)可知,AFB1對PBL之RNA合成傷 害作用無法再藉由BC而改善。C組和D組之蛋白質合成顯著較BC單獨添加對照組(E組)為低, BC(A組及B組)添加有改善AFB1對蛋白質合成抑制作用之趨勢。 實驗二、生體外BC添加對黃麴毒素B1所引發的豬肺泡巨噬細胞存活率及吞噬干擾作用 之影響。在存活率方面,同時間添加BC與AFB1(A組)顯著(P<0.05)較BC先與巨噬細胞培養2 小時後,再加入AFB1(B組)之細胞存活率高,而AFB1與巨噬細胞培養2小時後,再加入BC(C 組)其細胞存活率與單獨AFB1添加組(D組)相同,由此可知BC之添加應在AFB1加入培養系統 之前,否則無法發揮保護效果。關於吞噬作用方面,各組間均無差異顯著性,但培養系統中一 經添加有AFB1,吞噬作用之表現均有較其他各處理組為差之趨勢。 綜觀本實驗,BC(0.2μM/ml)可因添加之時機,對AFB1(5 ng/ml)所引發的豬周邊血液淋巴細 胞RNA和蛋白質合成及肺泡巨噬細胞存活率的抑制反應,具某種程度之保護效果。 |
英文摘要 | The purpose of this experiment was to investigate the protection of β-carotene (BC) on aflatoxin B1-induced (AFB1) swine peripheral blood lymphocyte (PBL) and alveolar macrophage toxicity in vitro. Trial 1: The effect of BC addition on DNA, RNA, and protein synthesis of swine PBL induced by AFB1 in vitro was studied. The DNA synthesis result showed that AFB1 (5 ng/ml) treated group (group D) had a slight tendency toward inhibition; however, there was no significant difference between the medium and AFB1 treated group, and the effect was slightly reduced when BC (0.2 μ g/ml) was added. The RNA synthesis was significantly inhibited by AFB1, but the inhibition became less evident when BC was added simultaneously with AFB1 (group A) or 2 hr before AFB1 introduced (group B). Thus, the time of addition of BC was critical to RNA synthesis. Based on results with BC introduced 2 hr after AFB1 addition (group C), it could be concluded that the detrimental effect of AFB1 on PBL's RNA synthesis could not be eliminated by BC addition. The protein synthesis of group C and group D was significantly lower than that of BC control (group E). The addition of BC (group A and B) tended to reduce the negative effect of AFB1. Trial 2: The effect of BC addition on cell viability and phagocytosis of macrophage induced by AFB1 in vitro was studied. For cell viability, coculture of BC with AFB1 (group A) was higher than AFB1 introduced 2 hr after BC addition (group B). BC introduced 2 hr after AFB1 addition (group C) had similar cell viability as that of AFB1 control (group D). Apparently, BC should be added to the culture before AFB1 to have a protective effect. For phagocytosis, there were no significant differences among these treatments, but AFB1 tended to decrease phagocytosis of macrophages. Overall, the inhibitory effect on swine PBL RNA, protein synthesis, and alveolar macrophage viability was induced by AFB1 (5×100 ng/ml). Depending on time of addition, BC (0.2 μM/ml) could exert some protection against some of the AFB1 -induced adverse effects. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。