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題 名 | 年齡、豬生殖與呼吸綜合症病毒及豬霍亂沙氏桿菌感染對豬肺泡巨噬細胞的表現型和功能之影響=Effect of Age, Porcine Reproductive and Respiratory Syndrome Virus and Salmonella choleraesuis Infection on the Phenotype and Phagocytic Activity of Porcine Alveolar Macrophages |
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作 者 | 廖偉莉; 簡茂盛; 劉正義; 林正忠; 謝快樂; 李維誠; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 25:3 1999.09[民88.09] |
頁 次 | 頁226-233 |
分類號 | 437.657 |
關鍵詞 | 肺泡巨噬細胞; 表現型; 吞噬作用; 豬霍亂沙氏桿菌; 豬生殖與呼吸綜合症; Alveolar macrophage; Phenotype; Phagocytosis; Salmonellosis; PRRS; |
語 文 | 中文(Chinese) |
中文摘要 | 肺泡巨噬細胞(pulmonary alveolar macrophages; PAM)在下呼吸道扮演著第一道防線,因此探索豬隻PAM之表現型與功能及病原對其之影響,有助於了解豬隻罹患肺炎之機制。本實驗選取健康哺乳小豬,於3週齡離乳後,隨即隔離飼養於實驗動物舍,並於不同生長階段,進行肺部灌洗,以流式細胞儀分析PAM之表現型及功能,並評估年齡因子對PAM之影響。將此基本資料與相同年齡層之豬生殖與呼吸綜合症病毒(porcine reproductive and respiratory syndrome virus; PRRSV)及敗血症型之豬霍亂沙氏桿菌(Salmonella choleraesuis)自然感染豬之PAM相比較。結果顯示,12週齡豬隻PAM之MHC class Ⅰ(anti-74-11-10 & 7-34-1)、MHC class Ⅱ(anti-MSA3)分子及抗巨噬細胞表面分子(anti-74-22-15 & 2G4a)之表現量顯著低於4及8週齡之豬隻(P<0.05),而4及8週齡間則無明顯差異。在對紅血球之吞噬功能及玫瑰作用方面,亦以4-8週齡功能較高,12週齡之豬隻則有下降之現象。對PRRSV自然感染豬而言,PAM之 MHC class I及抗巨噬細胞表面分子表現量及對RBC之玫瑰作用,均顯著低於相似年齡層之豬隻(P<0.05)。豬霍亂沙氏桿菌感染豬PAM之MHC class Ⅰ及巨噬細胞表面分子亦均顯著低於同齡的豬隻(P<0.05)。以上研究結果顯示年齡因子會影響到豬PAM表面分子表現及吞噬功能,在PRRSV自然感染下所呈現之對RBC之玫瑰作作降低現象,則反應PRRSV 感染下之肺微環境對病原之清除能力之被抑制狀態。 |
英文摘要 | Pulmonary alveolar macrophages (PAM) play an important role in clearing invading pathogens. The experiment was aimed to study the effect of age and pathogens on the phenotype expression and phagocytic activity of porcine PAM. Experimental pigs were weaned at 3-week-old and then raised in an experimental control house. Bronchoalveolar lavages were collected from pigs of different ages for phenotype and functional assays. The intensity of surface molecule expression and phagocytic activity of PAM were assayed by flow cytometry. No significant difference in the expression of MHC class I (monoclonal antibody, mAb, 74-11-10 & 7-34-1), MHC class Ⅱ (mAb MSA3), and macrophage markers (mAb 74-22-15 & 2G4a) of PAM was observed between 4- and 8-week-old pigs. However, the surface molecule expressions of PAM from 4- and 8-week-old pigs were significantly increased as compared to 12-week-old pigs (p<0.05). Similarly, a significant decrease in the RBC phagocytosis and rosetting activities of PAM from 12-week-old pigs was also observed as compared to the younger pigs (P<0.05). However surface molecule expressions of MHC class I and macrophage marker of PAM in Salmonella choleraesuis and porcine reproductive and respiratory syndrome (PRRS) virus infected pigs were significantly decreased (P<0.05). The RBC resetting activity of PAM from PRRS virus infected pigs was also significantly decreased when compared to pigs with the same age (p<0.05). Thus, age of the pigs may influence the phenotype and phagocytic activity of PAM. The decrease of RBC rosetting of PAM in PRRS virus infection may reflect a reduced state of pulmonary microenvironment in clearing invading pathogens. |
本系統中英文摘要資訊取自各篇刊載內容。