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題名 | Mitochondrial Dehydrogenase Depletion in Leaflet Matrix Cells during Processing of Cryopreserved Cardiac Valves=心臟瓣膜細胞活性與超低溫冷凍之關係 |
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作者姓名(中文) | 陸振翮; 張燕; 黃碧桃; 楊平政; 毛仁琰; | 書刊名 | Acta Cardiologica Sinica |
卷期 | 13:3 民86.07-09 |
頁次 | 頁117-122 |
分類號 | 416.262 |
關鍵詞 | 心臟瓣膜同種移植; 心臟膜; 冷凍保存; Homograft; Cardiac valve; Mitochondrial dehydrogenase; Cryopreservation; |
語文 | 英文(English) |
中文摘要 | 背景:超低溫冷凍心瓣膜在臨床上,使用已超過35年,因為心瓣膜捐贈取得不易,因此如何改善冷凍瓣膜之保存方法及減少瓣膜因組織缺氧而產生的細胞活性喪失是冷凍技術的關鍵。 方法:本實驗將156枚豬心瓣膜分為冷凍組(78枚)及非冷凍組(78枚),這兩組瓣膜分別置於4℃、24℃及37℃三種不同的溫度中保存,並放置使其組織缺氧時間分別為0、9、1、30及60小時,組織代謝的傷害程度,由其瓣膜內存留的粒腺體還原脢活性測定之。 結果:在非冷凍組織的瓣糢,經過60小時的組織缺氧保存4℃及24℃的瓣膜存有75%的活性,所有經過冷凍的瓣膜的活性皆較未冷凍者為低,如果將瓣膜保存於37℃培養營養機中60小時後,僅餘20%的活性。 結論:我們的結果顯示如果組織及早放於4℃及24℃之培養機中可以有效的減少冷凍傷害。 |
英文摘要 | Background. Cryopreserved cardiac valves in homograft have been clinically used for the past 35 years. Because of the shortage of donors, method of preservation and ischemia time-interval allowed have become critical steps in precryoprocessing. The injurous effects of preservation time and temperature of cardiac valves with and without cryopreservations have been studied. Methods. From 26 pigs, 156 semilunar leaflets were randomly divided into cryo-(n= 78) and noncryo- (n= 78) processed groups and harvested. For each preserving temperature of 4℃, 24℃, and 37℃, the leaflets were kept ischemically for 0, 9, 17, 30, and 60 hours, respectively. The metabolic injury to cardiac valves was determined by mitochondrial dehydrogenase (MD) activity. Results. The noncryoprocessed group retained about 75% activity of MD at 4 ℃ and 24 ℃ over a period of 60 hours. In the cryoprocessed group, the depletion of MD activity was more severe than for the non-cryoprocessed at all temperature tested. To maintain more than 50% of MD activity after cryoprocessing, short-term storage should be limited to 24 ℃ within 60 hours, 4℃ in 30 hours or 37℃ in 15 hours (p<0.01). Less than 20% activity of MD was found at 37℃ for 60 hours. Discussion. The results showed the significant influence of storage temperature on MD activity. Storage time interval is one of the critical factors in causing a steady loss of viability. When semilunar valves were maintained at 4℃ or 24℃, only minimal depletion of MD activity occurred under such "cadaveric" ischemia. |
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